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Investigation of a liposomal oxaliplatin drug formulation by capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry (CE-ICP-MS)

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Abstract

A capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) method was developed for separation of the free oxaliplatin drug substance from liposome-entrapped oxaliplatin. Simultaneous determination of phosphorous and platinum opened the possibility to simultaneously monitor the liposomes (phospholipids) and platinum-based drug. In order to suppress the interferences, argon gas was used as a collision gas in ICP-MS. A detection limit of 29 ng/mL of platinum and a precision of 2.9% (for 10 μg/mL of oxaliplatin standard) were obtained. Measurement of the total concentration of free and encapsulated oxaliplatin by CE-ICP-MS was compared with total determination by ICP-MS after microwave digestion and showed a good agreement. A liposomal formulation of oxaliplatin based on PEGylated liposomes was used as a model drug formulation. Studies of accelerated drug release induced by sonication and phospholipase A2 catalyzed hydrolysis were performed. It was demonstrated that the CE-ICP-MS was an efficient in vitro characterization method in the development and quality assurance purposes of lipsome-based formulation of metallodrugs.

Free and liposome-encapsulated platinum drug is distinguished by simultaneous detection of phosphorous and platinum by CE-ICP-MS

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Acknowledgments

LiPlasome Pharma A/S is gratefully acknowledged for the donation of phospholipase A2 and fruitful discussions.

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Authors and Affiliations

  1. Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark

    Tam T. T. N. Nguyen, Jesper Østergaard, Stefan Stürup & Bente Gammelgaard

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  1. Tam T. T. N. Nguyen
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  2. Jesper Østergaard
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  3. Stefan Stürup
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  4. Bente Gammelgaard
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Correspondence to Bente Gammelgaard.

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Nguyen, T.T.T.N., Østergaard, J., Stürup, S. et al. Investigation of a liposomal oxaliplatin drug formulation by capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry (CE-ICP-MS). Anal Bioanal Chem 402, 2131–2139 (2012). https://doi.org/10.1007/s00216-011-5651-6

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  • DOI: https://doi.org/10.1007/s00216-011-5651-6

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