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Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection

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Abstract

Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-functionalized silica nanoparticles was developed, in which Alexa Fluor 647 (AF647) molecules were covalently embedded. The coating of bovine serum albumin on the resultant fluorescent particles can greatly eliminate nonspecific background signal interference. The thus-synthesized fluorescent nanoparticles can specifically recognize biotin-labeled target DNA hybridized to the microarray via streptavidin–biotin interaction. The response of this DNA microarray technology exhibited a linear range within 0.2 to 10 pM complementary DNA and limit of detection of 0.1 pM, enhancing microarray hybridization sensitivity over tenfold. This promising technology may be potentially applied to other binding events such as specific interactions between proteins.

 

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Acknowledgements

This work was supported by Oklahoma Center for the Advancement of Science and Technology under Oklahoma Applied Research Support Program, and Oklahoma Bioenergy Center. A.L. thanks Chinese Academy of Sciences for honoring the Hundred-Talent-Project(KSCX2-YW-BR-7).

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Authors and Affiliations

  1. Institute for Environmental Genomics, Department of Botany and Microbiology, University of Oklahoma, Norman, OK, 73019, USA

    Aihua Liu, Liyou Wu, Zhili He & Jizhong Zhou

  2. Qingdao Institute of Bioenergy & Bioprocess Technology, and Key Laboratory of Bioenergy, Chinese Academy of Sciences, 189 Songling Road, Qingdao, Shandong, 266101, China

    Aihua Liu

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  1. Aihua Liu
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  2. Liyou Wu
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  3. Zhili He
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  4. Jizhong Zhou
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Correspondence to Aihua Liu, Liyou Wu or Jizhong Zhou.

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Liu, A., Wu, L., He, Z. et al. Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection. Anal Bioanal Chem 401, 2003–2011 (2011). https://doi.org/10.1007/s00216-011-5258-y

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  • DOI: https://doi.org/10.1007/s00216-011-5258-y

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