Abstract
Fourier-transform infrared (FTIR) imaging has been used to investigate brain tumor angiogenesis using a mice solid tumor model and bare-gold (∅ 25 nm) or BaSO4 (∅ 500 nm) nanoparticles (NP) injected into blood vasculature. FTIR images of 20-μm-thick tissue sections were used for chemical histology of healthy and tumor areas. Distribution of BaSO4-NP (using the 1,218–1,159 cm−1 spectral interval) revealed clearly all details of blood vasculature with morphological abnormalities of tumor capillaries, while Au-NP (using the 1,046–1,002 cm−1 spectral interval) revealed also diffusion properties of leaky blood vessels. Diffusion of Au-NP out of vascular space reached 64 ± 29 μm, showing the fenestration of “leaky” tumor blood vessels, which should allow small NP (<100 nm, as for Au-NP) to diffuse almost freely, while large NP should not (as for BaSO4-NP in this study). Therefore, we propose to develop FTIR imaging as a convenient tool for functional molecular histology imaging of brain tumor vasculature, both for identifying blood capillaries and for determining the extravascular diffusion space offered by vessel fenestration.
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Acknowledgments
The author is indebted to the contribution of “Association Française contre les Myopathies” (AFM—contract no. 14258), the “Ligue Nationale contre le cancer,” and the “Agence Nationale de la Recherche” (ANR—contract no. bl-inter09_464249—MIAG-X) for their financial supports. This research was also partially supported within the EU 7th Framework Programme (FP7/2007–2013) under the grant agreement no. 226716.
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Published in the special issue Imaging Techniques with Synchrotron Radiation with Guest Editor Cyril Petibois.
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Noreen, R., Pineau, R., Chien, CC. et al. Functional histology of glioma vasculature by FTIR imaging. Anal Bioanal Chem 401, 795–801 (2011). https://doi.org/10.1007/s00216-011-5069-1
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DOI: https://doi.org/10.1007/s00216-011-5069-1