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Kinetic analyses and performance of a colloidal magnetic nanoparticle based immunoassay dedicated to allergy diagnosis

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Abstract

In this paper, we demonstrate the possibility to use magnetic nanoparticles as immunosupports for allergy diagnosis. Most immunoassays used for immunosupports and clinical diagnosis are based on a heterogeneous solid-phase system and suffer from mass-transfer limitation. The nanoparticles’ colloidal behavior and magnetic properties bring the advantages of homogeneous immunoassay, i.e., species diffusion, and of heterogeneous immunoassay, i.e., easy separation of the immunocomplex and free forms, as well as analyte preconcentration. We thus developed a colloidal, non-competitive, indirect immunoassay using magnetic core–shell nanoparticles (MCSNP) as immunosupports. The feasibility of such an immunoassay was first demonstrated with a model antibody and described by comparing the immunocapture kinetics using macro (standard microtiter plate), micro (microparticles) and nanosupports (MCSNP). The influence of the nanosupport properties (surface chemistry, antigen density) and of the medium (ionic strength, counter ion nature) on the immunocapture efficiency and specificity was then investigated. The performances of this original MCSNP-based immunoassay were compared with a gold standard enzyme-linked immunosorbent assay (ELISA) using a microtiter plate. The capture rate of target IgG was accelerated 200-fold and a tenfold lower limit of detection was achieved. Finally, the MCSNP-based immunoassay was successfully applied to the detection of specific IgE from milk-allergic patient’s sera with a lower LOD and a good agreement (CV < 6%) with the microtiter plate, confirming the great potential of this analytical platform in the field of immunodiagnosis.

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References

  1. Hill DJ, Hosking CS (1996) Clin Exp Allergy 26:243–246

    Article  CAS  Google Scholar 

  2. Karlsson R, Michaelsson A, Mattson L (1991) J Immunol Meth 145:229–240

    Article  CAS  Google Scholar 

  3. Parsa H, Chin CD, Mongkolwisetwara P, Lee BW, Wang JL, Sia SK (2008) Lab Chip 8:2062–2070

    Article  CAS  Google Scholar 

  4. Lionello A, Josserand J, Jensen H, Girault HH (2005) Lab Chip 5:254–260

    Article  CAS  Google Scholar 

  5. Li HY, Dauriac V, Thibert V, Senechal H, Peltre G, Zhang XX, Descroix S (2010) Lab Chip 10:2597–2604

    Article  CAS  Google Scholar 

  6. Sato K, Tokeshi M, Kimura H, Kitamori T (2001) Anal Chem 73:1213–1218

    Article  CAS  Google Scholar 

  7. Ohashi T, Mawatari K, Sato K, Tokeshi M, Kitamori T (2009) Lab Chip 9:991–995

    Article  CAS  Google Scholar 

  8. Kim KS, Park JK (2005) Lab Chip 5:657–664

    Article  CAS  Google Scholar 

  9. Choi JW, Oh KW, Thomas JH, Heineman WR, Halsall HB, Nevin JH, Helmicki AJ, Henderson HT, Ahn CH (2002) Lab Chip 2:27–30

    Article  CAS  Google Scholar 

  10. Yang SY, Lien KY, Huang KJ, Lei HY, Lee GB (2008) Biosens Bioelectron 24:855–862

    Article  CAS  Google Scholar 

  11. Do J, Ahn CH (2008) Lab Chip 8:542–549

    Article  CAS  Google Scholar 

  12. Barbora J, Rosnerova S, Slovakova M, Zverinova Z, Hubalek M, Hernychova L, Rehulka P, Viovy JL, Bilkova Z (2008) J Chromatogr A 1206:64–71

    Article  Google Scholar 

  13. Yang Z, Liu H, Zong C, Yan F, Ju H (2009) Anal Chem 81:5484–5489

    Article  CAS  Google Scholar 

  14. Kwon Y, Hara CA, Knize MG, Hwang MH, Venkateswaran KS, Whellr EK, Bell PM, Renzi RF, Fruetel JA, Bailey CG (2008) Anal Chem 80:8416–8423

    Article  CAS  Google Scholar 

  15. Peyman SA, Iles A, Pamme N (2009) Lab Chip 9:3110–3117

    Article  CAS  Google Scholar 

  16. Chen HX, Busnel JM, Peltre G, Zhang XX, Girault HH (2008) Anal Chem 80:9583–9588

    Article  CAS  Google Scholar 

  17. Monte X, Funovics M, Montet-Abou K, Weissleder R, Josephson L (2006) J Med Chem 49:6087–6093

    Article  Google Scholar 

  18. Tassa C, Duffner JL, Lewis TA, Weissleder R, Schreiber ST, Koehler AN, Shaw SY (2010) Bioconjug Chem 21:14–19

    Article  CAS  Google Scholar 

  19. Hong S, Leroueil PR, Majoros IJ, Orr BG, Baker JR, Banaszak Holl MM (2007) Chem Biol 14:107–115

    Article  CAS  Google Scholar 

  20. Cedervall T, Lynch I, Lindman S, Berggard T, Thulin E, Nilsson H, Dawson KA, Linse S (2007) Proc Natl Acad Sci USA 104:2050–2055

    Article  CAS  Google Scholar 

  21. Lundqvist M, Stigler J, Elia G, Lynch I, Cedervall T, Dawson KA (2008) Proc Natl Acad Sci USA 105:14265–14270

    Article  CAS  Google Scholar 

  22. Gupta S, Huda S, Kilpatrick PK, Velve OD (2007) Anal Chem 79:3810–3820

    Article  CAS  Google Scholar 

  23. Bowma MC, Ballard TE, Ackerson CJ, Feldheim DL, Margolis DM, Melander C (2008) J Am Chem Soc 130:6896–6897

    Article  Google Scholar 

  24. Weissleder R, Kelly K, Sun EY, Shtatland T, Josephson L (2005) Nat Biotechnol 23:1418–1423

    Article  CAS  Google Scholar 

  25. Ibraimi F, Kriz D, Lu M, Hansson LO, Kriz K (2006) Anal Bioanal Chem 384:651–657

    Article  CAS  Google Scholar 

  26. Myszka DK (1997) Curr Opin Biotechnol 8:50–57

    Article  CAS  Google Scholar 

  27. Lin PC, Chou PH, Chen SH, Liao HK, Wang KY, Chen YJ, Lin CC (2006) Small 4:485–489

    Article  Google Scholar 

  28. Tang D, Yu Y, Niessner R, Miró M, Knopp D (2010) Analyst 135:2661–2667

    Article  CAS  Google Scholar 

  29. Liu X, Dai Q, Austin L, Coutts J, Knowles G, Zou JH, Chen H, Huo Q (2008) J Am Chem Soc 130:2780–2782

    Article  CAS  Google Scholar 

  30. Mao X, Baloda M, Gurung AS, Lin YH, Liu G (2008) Electrochem Comm 10:1636–1640

    Article  CAS  Google Scholar 

  31. Cai HH, Yang PH, Feng J, Cai JY (2009) Sens Act B 135:603–609

    Article  Google Scholar 

  32. Ye CH, Hung CY, Chang TC, Lin HP, Lin YC (2009) Microfluid Nanofluid 6:85–91

    Article  Google Scholar 

  33. Maurice V, Georgelin T, Siaugue JM, Cabuil V (2009) J Magn Magn Mater 321:1408–1413

    Article  CAS  Google Scholar 

  34. Georgelin T, Maurice V, Malezieux B, Siaugue JM, Cabuil V (2010) J Nanopart Res 12:675–680

    Article  CAS  Google Scholar 

  35. Georgelin T, Bombard S, Siaugue JM, Cabuil V (2010) Angew Chem 49:9091–9085

    Google Scholar 

  36. d’Orlyé F, Varenne A, Georgelin T, Siaugue JM, Teste B, Descroix S, Gareil P (2009) Electrophoresis 30:2572–2582

    Article  Google Scholar 

  37. Petr J, Teste B, Descroix S, Siaugue JM, Gareil P (2010) Varenne A Electrophor 31:2754–2761

    Article  CAS  Google Scholar 

  38. Teste B, Vial J, Descroix S, Georgelin T, Siaugue JM, Petr J, Varenne A, Hennion MC (2010) Talanta 81:1703–1710

    Article  CAS  Google Scholar 

  39. Bourdillon C, Demaille C, Moiroux J, Savéant JM (1999) J Am Chem Soc 121:2401–2408

    Article  CAS  Google Scholar 

  40. Cornec M, Cho D, Narsimhan G (1999) J Coll Interf Sci 214:129–142

    Article  CAS  Google Scholar 

  41. Amatore C, Fosset B (1996) Anal Chem 68:4377–4388

    Article  CAS  Google Scholar 

  42. Bard AJ, Faulkner LR (2001) Electrochemical methods, fundamentals and applications, 2nd edn. Wiley, New York

    Google Scholar 

  43. Xiao X, Bard AJ (2007) J Am Chem Soc 129:9610–9612

    Article  CAS  Google Scholar 

  44. Hsieh HV, Thompson NL (1995) Biochemistry 34:12481–12488

    Article  CAS  Google Scholar 

  45. Wandersman E, Dupuis V, Dubois E, Perzynski R (2009) Phys Rev E 80:041504–041512

    Article  CAS  Google Scholar 

  46. Krishnamurthy S, Bhattacharya P, Phelan PE, Prasher RS (2006) NanoLett 6:419–423

    CAS  Google Scholar 

  47. Northrup S, Erickson H (1992) Proc Natl Acad Sci USA 89:3338–3342

    Article  CAS  Google Scholar 

  48. Voss EW (1993) J Mol Recognit 6:51–58

    Article  CAS  Google Scholar 

  49. Baudry J, Rouzeau C, Goubault C, Robic C, Cohen-Tannoudji L, Koenig A, Bertrand E, Bibette J (2006) Proc Natl Acad Sci USA 103:16076–16080

    Article  CAS  Google Scholar 

  50. Cohen-Tannoudji L, Bertrand E, Baudry J, Robic C, Goubault C, Pellisier M, Johner A, Thalmann F, Lee NK, Marques CM, Bibette (2008) J Phys Rev Lett 100:108301–108304

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by the French research agency ANR SOLUDIAG ANR-07-BLAN-02660.

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Authors and Affiliations

  1. Physicochimie des Electrolytes, Colloïdes et Sciences Analytiques (PECSA) UMR 7195 CNRS-ESPCI-UPMC, 10 rue Vauquelin, 75231, Paris Cedex 05, France

    Bruno Teste, Frédéric Kanoufi, Stéphanie Descroix, Pascal Poncet & Marie-Claire Hennion

  2. CNRS-UPMC-ESPCI, 4 place Jussieu, 75252, Paris Cedex 05, France

    Thomas Georgelin & Jean-Michel Siaugue

  3. CNRS-ENSCP-UPMC, Ecole Nationale Supérieure de Chimie de Paris, Chimie Paris Tech, 11 rue Pierre et Marie Curie, 75231, Paris Cedex 05, France

    Jan Petr & Anne Varenne

  4. Institut Pasteur, 25–28 rue du Docteur Roux, 75015, Paris, France

    Pascal Poncet

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  1. Bruno Teste
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  2. Frédéric Kanoufi
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  3. Stéphanie Descroix
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  4. Pascal Poncet
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  5. Thomas Georgelin
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  6. Jean-Michel Siaugue
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  7. Jan Petr
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  8. Anne Varenne
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  9. Marie-Claire Hennion
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Correspondence to Stéphanie Descroix.

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Teste, B., Kanoufi, F., Descroix, S. et al. Kinetic analyses and performance of a colloidal magnetic nanoparticle based immunoassay dedicated to allergy diagnosis. Anal Bioanal Chem 400, 3395–3407 (2011). https://doi.org/10.1007/s00216-011-5021-4

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  • DOI: https://doi.org/10.1007/s00216-011-5021-4

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