Abstract
This article deals with the first covalent grafting of an enzyme on twice functionalized γ-Fe2O3@SiO2 core–shell magnetic nanoparticles. First, amino-PEG functionalized nanoparticles were synthesized in order to comply with non-toxic platforms that would be stable in high concentration and would exhibit chemical groups to allow further coupling with biomolecules. This approach produces a colloidal suspension of covalently grafted enzymes that remains stable for months and mimics the enzyme–substrate interactions in solution. Secondly, nanoparticles synthesis and enzyme coupling process were reported and the catalytic properties of bound enzymes were measured and compared with that of the free one. These new materials appear to be useful tools for enzymatic catalysis research and may be extended to other biomolecules. Furthermore, magnetic properties of these materials open the way to separation, purification, and transport under magnetic field.
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Acknowledgment
The authors would like to thank Delphine Talbot and Aude Michel for technical assistance.
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Georgelin, T., Maurice, V., Malezieux, B. et al. Design of multifunctionalized γ-Fe2O3@SiO2 core–shell nanoparticles for enzymes immobilization. J Nanopart Res 12, 675–680 (2010). https://doi.org/10.1007/s11051-009-9757-0
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DOI: https://doi.org/10.1007/s11051-009-9757-0