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Simultaneous profiling of lysophospholipids and phospholipids from human plasma by nanoflow liquid chromatography-tandem mass spectrometry

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Abstract

In this study, an analytical method for the simultaneous separation and characterization of various molecular species of lysophospholipids (LPLs) and phospholipids (PLs) is introduced by employing nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC-ESI-MS/MS). Since LPLs and PLs in human plasma are potential biomarkers for cancer, development of a sophisticated analytical method for the simultaneous profiling of these molecules is important. Standard species of LPLs and PLs were examined to establish a separation condition using a capillary LC column followed by MS scans and data-dependent collision-induced dissociation (CID) analysis for structural identification. With nLC-ESI-MS/MS, regioisomers of each category of LPLs were completely separated and identified with characteristic CID spectra. It was applied to the comprehensive profiling of LPLs and PLs from a human blood plasma sample and yielded identifications of 50 LPLs (each regioisomer pair of 6 lysophosphatidylcholines (LPCs), 7 lysophosphatidylethanolamines (LPEs), 9 lysophosphatidic acid (LPAs), 2 lysophosphatidylglycerols (LPGs), and 1 lysophosphatidylserine (LPS)) and 62 PLs (19 phosphatidylcholines (PCs), 11 phosphatidylethanolamines (PEs), 3 phosphatidylserines (PSs), 16 phosphatidylinositols (PIs), 8 phosphatidylglycerols (PGs), and 5 phosphatidic acids (PAs)).

The study demonstrates that regioisomers of lysophospholipid can be completely separated and identified with characteristic CID spectra using nLC-ESI-MS-MS, along with the simultaneous profiling of phospholipids from human blood plasma.

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Acknowledgements

This study was supported by a grant (NRF-2010-0014046) from the National Research Foundation of Korea.

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  1. Department of Chemistry, Yonsei University, Seoul, 120-749, South Korea

    Ju Yong Lee, Hye Kyeong Min & Myeong Hee Moon

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  1. Ju Yong Lee
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  2. Hye Kyeong Min
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  3. Myeong Hee Moon
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Correspondence to Myeong Hee Moon.

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Lee, J.Y., Min, H.K. & Moon, M.H. Simultaneous profiling of lysophospholipids and phospholipids from human plasma by nanoflow liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem 400, 2953–2961 (2011). https://doi.org/10.1007/s00216-011-4958-7

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  • DOI: https://doi.org/10.1007/s00216-011-4958-7

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