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Quantitative analysis of phosphatidylcholines and phosphatidylethanolamines in urine of patients with breast cancer by nanoflow liquid chromatography/tandem mass spectrometry

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Abstract

Phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) from urine of patients with breast cancer were qualitatively and quantitatively analyzed by nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC-ESI-MS-MS). Urinary phospholipids (PLs) were extracted from three different categories of patients (non-cancer controls and breast cancer patients before and after surgery) by first lyophilizing only 1 mL of urine sample to enrich PLs. Next, nLC-ESI-MS-MS analysis of intact urinary phospholipids was performed, resulting in structural identification of 21 PCs and 12 PEs, followed by quantitative analysis using a multiple standard addition method. This study demonstrated that nLC-ESI-MS-MS can be powerfully utilized for the study of relative changes in the contents and concentration of urinary PCs and PEs from breast cancer patients: total concentration of PCs and PEs of patient sample increased to (144 ± 9)% and (171 ± 11)%, respectively, compared to control sample but they decreased significantly following surgery.

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Acknowledgements

This study was supported by a grant (2008-03136) from the Korean Ministry of Science and Technology (MOST) and partly by a grant from the Korean Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH).

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Authors and Affiliations

  1. Department of Chemistry, Yonsei University, Seoul, 120-749, South Korea

    Hanna Kim, Hye Kyeong Min & Myeong Hee Moon

  2. Department of Pathology, College of Medicine, Hanyang University, Seoul, 133-791, South Korea

    Gu Kong

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  1. Hanna Kim
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  2. Hye Kyeong Min
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  3. Gu Kong
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  4. Myeong Hee Moon
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Correspondence to Myeong Hee Moon.

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Kim, H., Min, H.K., Kong, G. et al. Quantitative analysis of phosphatidylcholines and phosphatidylethanolamines in urine of patients with breast cancer by nanoflow liquid chromatography/tandem mass spectrometry. Anal Bioanal Chem 393, 1649–1656 (2009). https://doi.org/10.1007/s00216-009-2621-3

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  • DOI: https://doi.org/10.1007/s00216-009-2621-3

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