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High-resolution, imaging TOF-SIMS: novel applications in medical research

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Abstract

The use of high-resolution, imaging TOF-SIMS is described and examples are made to demonstrate the application of the method in medical research. Cytochemistry by TOF-SIMS is shown by localization of diacylglycerol (DG) in cryostat sections of hyaline cartilage and by localization of corticosterone in cryostat sections of the adrenal gland cortex. Quantitative measurements and comparison of groups is shown by comparing the lipid content of adipose tissue from two mouse strains, transgenic mouse expressing the FOXC2 gene and wild-type controls. Finally, biopsies made for histopathological diagnosis of infantile reversible cytochrome c oxidase deficiency myopathy were analyzed in order to define the chemical content of areas showing a pathological structure in the light microscope. The use of high-resolution, imaging TOF-SIMS in medical research allows analysis of intact tissue and probe-free localization of specific target molecules in cells and tissues. The TOF-SIMS analysis is not dependent on penetration of reagents into the sample and also independent of probe reactivity such as cross-reactivity or background staining. The TOF-SIMS method can be made quantitative and allows for analysis of specific target molecules in defined tissue compartments.

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Authors and Affiliations

  1. Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, P.O. Box 100, 405 30, Göteborg, Sweden

    Per Malmberg, Eva Jennische & Håkan Nygren

  2. Department of Medical Genetics, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Box 100, 405 30, Göteborg, Sweden

    Daniel Nilsson

  3. Institute of Biomedicine, P.O. Box 420, 405 30, Göteborg, Sweden

    Håkan Nygren

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  1. Per Malmberg
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  2. Eva Jennische
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  3. Daniel Nilsson
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  4. Håkan Nygren
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Correspondence to Håkan Nygren.

Additional information

This paper was published in the special issue on Advances in Analytical Mass Spectrometry with Guest Editor Maria Careri.

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Malmberg, P., Jennische, E., Nilsson, D. et al. High-resolution, imaging TOF-SIMS: novel applications in medical research. Anal Bioanal Chem 399, 2711–2718 (2011). https://doi.org/10.1007/s00216-010-4155-0

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  • DOI: https://doi.org/10.1007/s00216-010-4155-0

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