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Mass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF–SIMS

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Abstract

Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different techniques are now available: matrix-assisted laser desorption/ionizaton, where the sample, preliminary coated by an organic matrix, is analyzed by a UV laser beam; and secondary ion mass spectrometry, for which the target is directly submitted to a focused ion beam. Both techniques revealed excellent performances for lipid mapping of tissue surfaces. This article will discuss similarities, differences, and specificities of ion images generated by these two techniques in terms of sample preparation, sensitivity, ultimate spatial resolution, and structural analysis.

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Acknowledgments

This work and the PhD research fellowship of FB are supported by the European Union (contract LSHG-CT2005-518194 COMPUTIS). The PhD research fellowship of AS is supported by the Institut de Chimie des Substances Naturelles (ICSN-CNRS). This work is also supported by the French Agence Nationale pour la Recherche (EICO-CF, grant no. ANR-07-MRAR-006-01).

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Authors and Affiliations

  1. Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France

    Farida Benabdellah, Alexandre Seyer, David Touboul, Alain Brunelle & Olivier Laprévote

  2. Laboratoire de spectrométrie de masse, Centre d’Analyse des Résidus en Traces—GIGA-R, Université de Liège, 4000, Liège, Belgium

    Loïc Quinton

  3. IFR 71, Faculté de Pharmacie, Université Paris Descartes, 4 Avenue de l’Observatoire, 75006, Paris, France

    Olivier Laprévote

Authors
  1. Farida Benabdellah
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  2. Alexandre Seyer
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  4. David Touboul
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  5. Alain Brunelle
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  6. Olivier Laprévote
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Corresponding author

Correspondence to David Touboul.

Additional information

Farida Benabdellah and Alexandre Seyer contributed equally to this work.

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Benabdellah, F., Seyer, A., Quinton, L. et al. Mass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF–SIMS. Anal Bioanal Chem 396, 151–162 (2010). https://doi.org/10.1007/s00216-009-3031-2

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  • DOI: https://doi.org/10.1007/s00216-009-3031-2

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