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Determination of selenomethionine, selenocysteine, and inorganic selenium in eggs by HPLC–inductively coupled plasma mass spectrometry

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Abstract

A method for the simultaneous determination of selenomethionine (SeMet), selenocysteine (SeCys), and selenite [Se(IV)] in chicken eggs was developed. A sample preparation protocol including defatting, protein denaturation, and carbamidomethylation was optimized in order to achieve complete protein digestion and to avoid SeCys losses. Quantification was carried out by reversed-phase HPLC–inductively coupled plasma mass spectrometry (ICP MS) after quantitative isolation of the selenium-containing fraction by size-exclusion liquid chromatography. The detection limits were 0.06, 0.003, and 0.01 µg g−1 (dry weight) for SeCys, Se(IV) and SeMet, respectively, and the precision was 5–10%. The end products of carbamidomethylation of the different selenium species were identified for the first time by electrospray QTOF MS after custom-designed 2D HPLC purification. Differences in selenium speciation in egg yolk and white were highlighted, the yolk containing more SeCys and the white more SeMet. An insight into selenium bioaccessibility in eggs was obtained by digestion with simulated gastric and gastrointestinal juices and size-exclusion HPLC-ICP MS.

Selenium speciation in chicken eggs

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Acknowledgments

The authors thank Dr. Gérard Bertin Alltech, France, for facilitating the acquisition of the authentic control and supplemented eggs and Prof. Dr. Ryszard Lobinski, CNRS, Pau, for the critical reading of the manuscript. This work was supported by the EU in the framework of European Social Fund through the Warsaw University of Technology Development Programme.

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Authors and Affiliations

  1. CNRS/UPPA, Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, UMR 5254, Hélioparc, 2, av. Pr. Angot, 64053, Pau, France

    Elżbieta Lipiec, Grzegorz Siara, Katarzyna Bierla, Laurent Ouerdane & Joanna Szpunar

  2. Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland

    Elżbieta Lipiec & Grzegorz Siara

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  1. Elżbieta Lipiec
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  2. Grzegorz Siara
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  3. Katarzyna Bierla
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  4. Laurent Ouerdane
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  5. Joanna Szpunar
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Correspondence to Joanna Szpunar.

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Lipiec, E., Siara, G., Bierla, K. et al. Determination of selenomethionine, selenocysteine, and inorganic selenium in eggs by HPLC–inductively coupled plasma mass spectrometry. Anal Bioanal Chem 397, 731–741 (2010). https://doi.org/10.1007/s00216-010-3544-8

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  • DOI: https://doi.org/10.1007/s00216-010-3544-8

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