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Thiol-stabilized luminescent CdTe quantum dot as biological fluorescent probe for sensitive detection of methyl parathion by a fluoroimmunochromatographic technique

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Abstract

Quantum dots (QDs) are preferred as high-resolution biological fluorescent probes because of their inherent optical properties compared with organic dyes. This intrinsic property of QDs has been made use of for sensitive detection of methylparathion (MP) at picogramme levels. The specificity of the assay was attributed to highly specific immunological reactions. Competitive binding between free MP and CdTe QD bioconjugated MP (MP-BSA-CdTe) with immobilized anti-MP IgY antibodies was monitored in a flow-injection system. The fluorescence intensity of MP-BSA-CdTe bioconjugate eluted from the column was found to be directly proportional to the free MP concentration. Hence, it was possible to detect MP in a linear range of 0.1–1 ng mL−1 with a regression coefficient R 2 = 0.9905. In this investigation, IgY proved advantageous over IgG class immunoglobulins in terms of yield, stability, cost effectiveness, and enhancement of assay sensitivity. The photo-absorption spectrum of bioconjugated CdTe QD (λ max = 310 nm) confirmed nano-biomolecular interactions. The results suggest the potential application of bioconjugation and nano-biomolecular interactions of QDs for biological labeling and target analyte detection with high sensitivity.

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Acknowledgement

The authors thank the Director, CFTRI, Mysore, for providing facilities. Mr R.S. Chouhan and Mr A.C. Vinayaka are grateful to the Council of Scientific and Industrial Research (CSIR) for Senior Research Fellowships. The Department of Biotechnology, Government of India, and the Swiss Development Cooperation, Switzerland, are gratefully acknowledged for providing financial support under the Indo-Swiss Biotechnology collaborative project (ISCB). Dr K.R. Thampi, Dr Sridevi A. Singh, Dr P. Srinivas, Dr K. N. Gurudutt, and Dr Sanjukta Patra, IIT, Gowahati are acknowledged for their kind help and support.

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Authors and Affiliations

  1. Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute (A constituent laboratory of Council of Scientific and Industrial Research, New Delhi), Mysore, 570020, India

    Raghuraj Singh Chouhan, Aaydha Chidambara Vinayaka & Munna Singh Thakur

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  1. Raghuraj Singh Chouhan
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  2. Aaydha Chidambara Vinayaka
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  3. Munna Singh Thakur
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Correspondence to Munna Singh Thakur.

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Chouhan, R.S., Vinayaka, A.C. & Thakur, M.S. Thiol-stabilized luminescent CdTe quantum dot as biological fluorescent probe for sensitive detection of methyl parathion by a fluoroimmunochromatographic technique. Anal Bioanal Chem 397, 1467–1475 (2010). https://doi.org/10.1007/s00216-009-3433-1

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  • DOI: https://doi.org/10.1007/s00216-009-3433-1

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