Skip to main content

Advertisement

SpringerLink
Log in

Fluorescent turn-off competitive immunoassay for biotin based on hydrothermally synthesized carbon dots

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

Blue fluorescent carbon dots (C-Dots) were synthesized by a hydrothermal process using citric acid and polyethylene-polyamines as the precursors. The C-dots display strong fluorescence, excellent water solubility, good biocompatibility, and possess many amino groups on their surface. Biotin was linked to the amino groups, and the resulting biotinylated C-dots were applied in a binding assay where they compete with free biotin (vitamin B7; coenzyme R) for binding to streptavidin immobilized on superparamagnetic Dynabeads®. After incubation and magnetic separation, the fluorescence intensity of immobilized biotinylated C-dots released from the beads is inversely proportional to the concentration of biotin in the 0.5 to 100 ng·mL−1 concentration range. The detection limit is 0.1 ng·mL−1. Conceivably, the method may be further extended to the determination of biotinylated biomolecules.

One-pot hydrothermal synthesized C-Dots were biotinylated and used as fluorescent probe in the immunoassay for the determination of Vitamin B7.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Scheme 1
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Lin Y, Yao B, Huang T, Zhang S, Cao X, Weng W (2016) Selective determination of free dissolved chlorine using nitrogen-doped carbon dots as a fluorescent probe. Microchim Acta 183(7):2221–2227

    Article  CAS  Google Scholar 

  2. Li X, Zhang S, Kulinich SA, Liu Y, Zeng H (2014) Engineering surface states of carbon dots to achieve controllable luminescence for solid-luminescent composites and sensitive Be2+ detection. Sci Rep 4:1–8

    Google Scholar 

  3. Yang ST, Cao L, Luo PG, Lu F, Wang X, Wang H, Meziani MJ, Liu Y, Qi G, Sun YP (2009) Carbon dots for optical imaging in vivo. J Am Chem Soc 131:11308–11309

    Article  CAS  Google Scholar 

  4. Yulong Y, Xinsheng P (2016) Recent advances in carbon-based dots for electroanalysis. Analyst 141:2619–2628

    Article  Google Scholar 

  5. Lim SY, Shen W, Gao Z (2015) Carbon quantum dots and their applications. Chem Soc Rev 44:362–381

    Article  CAS  Google Scholar 

  6. Wang W, Lu YC, Huang H, Feng JJ, Chen JR, Wang AJ (2014) Facile synthesis of water-soluble and biocompatible fluorescent nitrogen-doped carbon dots for cell imaging. Analyst 139:1692–1696

    Article  CAS  Google Scholar 

  7. Hola K, Zhang Y, Wang Y, Giannelis EP, Zboril R, Rogach AL (2014) Carbon dots-emerging light emitters for bioimaging, cancer therapy and optoelectronics. Nano Today 9:590–603

    Article  CAS  Google Scholar 

  8. Najafabadi AT, Gyenge E (2014) High-yield graphene production by electrochemical exfoliation of graphite: novel ionic liquid (IL)–acetonitrile electrolyte with low IL content. Carbon 71:58–69

    Article  CAS  Google Scholar 

  9. Gonçalves H, Jorge PAS, Fernandes JRA, Esteves da Silva JCG (2010) Hg(II) sensing based on functionalized carbon dots obtained by direct laser ablation. Sensor Actuat B-Chem 145:702–707

    Article  Google Scholar 

  10. Jiang Y (2015) N-doped carbon dots synthesized by rapid microwave irradiation as highly fluorescent probes for Pb2+ detection. New J Chem 39:3357–3360

    Article  CAS  Google Scholar 

  11. Wu Y, Wei P, Pengpumkiat S, Schumacher EA, Remcho VT (2015) Development of a carbon dot (C-dot)-linked immunosorbent assay for the detection of human α-fetoprotein. Anal Chem 87:8510–8516

    Article  CAS  Google Scholar 

  12. Liu H, Wang Q, Shen G, Zhang C, Li C, Ji W, Wang C, Cui D (2014) A multifunctional ribonuclease A-conjugated carbon dot cluster nanosystem for synchronous cancer imaging and therapy. Nanoscale Res Lett 9:1–11

    Article  Google Scholar 

  13. Mohapatra S, Sahu S, Sinha N, Bhutia SK (2014) Synthesis of a carbon-dot-based photoluminescent probe for selective and ultrasensitive detection of Hg(2+) in water and living cells. Analyst 140:1221–1228

    Article  Google Scholar 

  14. Yuan Y, Liu Z, Li R, Zou H, Lin M, Liu H, Huang C (2016) Synthesis of nitrogen-doping carbon dots with different photoluminescence properties by controlling the surface states. Nanoscale 8:294–297

    Google Scholar 

  15. Bailey LM, Ivanov RA, Wallace JC, Polyak SW (2008) Artifactual detection of biotin on histones by streptavidin. Anal Biochem 373:71–77

    Article  CAS  Google Scholar 

  16. Otten JJ, Hellwig JP, Meyers LD, Medicine IO (2006) DRI, dietary reference intakes: the essential guide to nutrient requirements. Am J Clin Nutr

    Google Scholar 

  17. Huang JL, Yang ZW (2015) Microorganism determination of biotin content in multi-vitamins tablets. J Food Saf Qual 6(8):3045–3049

    Google Scholar 

  18. Thompson LB, Schmitz DJ, Pan SJ (2006) Determination of biotin by high-performance liquid chromatography in infant formula, medical nutritional products, and vitamin premixes. J AOAC Int 89:1515–1518

    CAS  Google Scholar 

  19. Wang FL, Liu AG, Sun JJ, Peng C (2013) Simultaneous determination of folic acid, Vitamin B12 and biotin in infant formula by HPLC-MS-MS. Food Sci 34: 269–272

  20. Sun MJ, Chen YS, Zhang XW, Chen Q (2014) Detection of biotin in milk powder basing on surface plasmon resonance. J Food Saf Qual 5:1724–1727

    Google Scholar 

  21. Schray KJ, Artz PG, Hevey RC (1988) Determination of avidin and biotin by fluorescence polarization. Anal Chem 60:853–855

    Article  CAS  Google Scholar 

  22. Wilchek M, Bayer EA (1988) The avidin-biotin complex in bioanalytical applications. Anal Biochem 171:1–32

    Article  CAS  Google Scholar 

  23. Wilchek M, Bayer EA (1984) The avidin-biotin complex in immunology. Immunol Today 5:39–43

    Article  CAS  Google Scholar 

  24. Gajos K, Petrou P, Budkowski A, Awsiuk K, Bernasik A, Misiakos K, Rysz J, Raptis I, Kakabakos S (2014) Imaging and spectroscopic comparison of multi-step methods to form DNA arrays based on the biotin-streptavidin system. Analyst 140:1127–1139

    Article  Google Scholar 

  25. Dundas CM, Demonte D, Park S (2013) Streptavidin-biotin technology: improvements and innovations in chemical and biological applications. Appl Microbiol Biotechnol 97:9343–9353

    Article  CAS  Google Scholar 

  26. Stayton PS, Freitag S, Klumb LA, Chilkoti A, Chu V, Penzotti JE, To R, Hyre D, Trong IL, Lybrand TP (1999) Streptavidin–biotin binding energetics. Biomol Eng 16:39–44

    Article  CAS  Google Scholar 

  27. Srisa-Art M, Dyson EC, Demello AJ, Edel JB (2008) Monitoring of real-time streptavidin-biotin binding kinetics using droplet microfluidics. Anal Chem 80:7063–7067

    Article  CAS  Google Scholar 

  28. Wilson R (2011) Preparation of single-stranded DNA from PCR products with streptavidin magnetic beads. Nucleic Acid Ther 21:437–440

    Article  CAS  Google Scholar 

  29. Björner M, Bergh A, Glad G, Granéer T, Hedlund H, Hellberg U, Uhléen K (2012) High performance protein enrichment using streptavidin magnetic beads. J Biomol Tech 23:S30

    Google Scholar 

  30. Anders H, Anna B, Olof N, Morten L, Joakim L, Mathias U (2005) The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures. Electrophoresis 26:501–510

    Article  Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the financial support of National Natural Science Foundation of China (Nos. 21305015, Nos. 21275028), National Science Foundation for Distinguished Young Scholars of Fujian Province (Nos. 2016 J06019), Natural Science Foundation of Fujian Province of China (Nos. 2014 J05014, 2014 J07009), Joint Funds for the innovation of science and Technology(Fujian province, Nos. 2016Y91010007), Program for Fujian University Outstanding Youth Scientific Research (Nos. 2015b026), and Program for Fujian Top-notch Innovative Personnel.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350004, China

    Wensong Yao, Namei Wu, Zhen Lin, Jinyuan Chen, Shaoguang Li, Shaohuang Weng, Lingling Zhang, Ailin Liu & Xinhua Lin

  2. Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, 350004, China

    Wensong Yao, Zhen Lin, Jinyuan Chen, Shaoguang Li, Shaohuang Weng, Lingling Zhang, Ailin Liu & Xinhua Lin

  3. Department of Pharmacy, The Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou, 362000, China

    Namei Wu

Authors
  1. Wensong Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Namei Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhen Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jinyuan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shaoguang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shaohuang Weng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lingling Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ailin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Xinhua Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Zhen Lin or Xinhua Lin.

Ethics declarations

The author(s) declare that they have no competing interests

Additional information

The first two authors Wensong Yao and Namei Wu contribute equally to the present manuscript.

Electronic supplementary material

ESM 1

(DOC 198 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yao, W., Wu, N., Lin, Z. et al. Fluorescent turn-off competitive immunoassay for biotin based on hydrothermally synthesized carbon dots. Microchim Acta 184, 907–914 (2017). https://doi.org/10.1007/s00604-017-2078-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-017-2078-6

Keywords

Search

Navigation