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Synchrotron FTIR microspectroscopy of the yeast Saccharomyces cerevisiae after exposure to plasma-deposited nanosilver-containing coating

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Abstract

The present work was focused on elucidating changes in the model yeast Saccharomyces cerevisiae (cell composition, ultrastructure) after exposure to antimicrobial plasma-mediated nanocomposite films. In order to achieve this, a nanosilver-containing coating was deposited onto stainless steel using radiofrequency HMDSO plasma deposition, combined with simultaneous silver sputtering. X-ray photoelectron spectroscopy (XPS) confirmed the presence of silver nanoparticles embedded in an organosilicon matrix. In addition, scanning electron microscopy (SEM) demonstrated the nanoparticle-based morphology of the deposited layer. The antifungal properties towards S. cerevisiae were established, since a 1.4 log reduction in viable counts was observed after a 24-h adhesion compared to control conditions with the matrix alone. Differences in cell composition after exposure to the nanosilver was assessed for the protein region using, for the first time, synchrotron Fourier-transform infrared (FTIR) microspectroscopy of single S. cerevisiae cells, through in situ mapping with sub-cellular spatial resolution. IR spectrum of yeast cells recovered after a 24-h adhesion to the nanosilver-containing coating revealed a significant downshift (20 cm−1) of the amide I peak at 1655 cm−1, compared to freshly harvested cells. This lower band position, corresponding to a loss in α-helix structures, is indicative of the disordered secondary structures of proteins, due to the transition between active and inactive conformations under nanosilver-induced stress conditions. No significant effect on the nucleic acid region was detected. The inhibitory action of silver was targeted against both cell wall and intracellular proteins such as enzymes. Transmission electron microscopy (TEM) observations of the yeast ultrastructure confirmed serious morphological and structural damages. A homogeneous protein-binding distribution of nanosilver all over the cell was assumed, since the presence of electron-dense silver clusters was detected not only on the cell surface but also within the cell. For control experiments with the organosilicon matrix alone, no antimicrobial effect was observed, which was consistent with synchrotron FTIR results and TEM observations.

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Acknowledgements

The research described in this paper was performed at the French national synchrotron facility SOLEIL (Gif-sur-Yvette, France), using the SMIS beamline (proposal no 20060176). The authors gratefully acknowledge the “BioPleasure” project of the Research National Agency (ANR-07-BLAN-0196-01) for funding. The authors wish to thank S. Zanna (LPCS, Paris, France) for XPS analysis. Thanks are also due to S. Le Blond du Plouy of the Electron Microscopy Centre TEMSCAN (Paul Sabatier University, Toulouse, France) for SEM images.

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Authors and Affiliations

  1. Université de Toulouse, INSA, UPS, INPT, LISBP, 135 Av. de Rangueil, 31077, Toulouse, France

    C. Saulou, C. Maranges & M. Mercier-Bonin

  2. INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, 31400, Toulouse, France

    C. Saulou, C. Maranges & M. Mercier-Bonin

  3. CNRS, UMR5504, 31400, Toulouse, France

    C. Saulou, C. Maranges & M. Mercier-Bonin

  4. Université de Toulouse, UPS, INPT, LAPLACE, 118 route de Narbonne, 31062, Toulouse cedex 9, France

    C. Saulou, B. Despax & P. Raynaud

  5. CNRS, LAPLACE, 31062, Toulouse, France

    C. Saulou, B. Despax & P. Raynaud

  6. INRA-Département CEPIA, Rue de la Géraudière, BP 71627, 44316, Nantes cedex 3, France

    F. Jamme

  7. Synchrotron SOLEIL, L’Orme des Merisiers, BP 48, Saint Aubin, 91192, Gif-sur-Yvette, France

    F. Jamme & P. Dumas

  8. Université Paul Sabatier, Centre de Microscopie Electronique Appliquée à la Biologie (CMEAB), Route de Narbonne, 31077, Toulouse cedex 4, France

    I. Fourquaux

  9. Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés, INSA, 135 Avenue de Rangueil, 31077, Toulouse cedex 4, France

    M. Mercier-Bonin

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  1. C. Saulou
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  2. F. Jamme
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  3. C. Maranges
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  4. I. Fourquaux
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  5. B. Despax
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  6. P. Raynaud
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  7. P. Dumas
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  8. M. Mercier-Bonin
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Correspondence to M. Mercier-Bonin.

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Saulou, C., Jamme, F., Maranges, C. et al. Synchrotron FTIR microspectroscopy of the yeast Saccharomyces cerevisiae after exposure to plasma-deposited nanosilver-containing coating. Anal Bioanal Chem 396, 1441–1450 (2010). https://doi.org/10.1007/s00216-009-3316-5

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  • DOI: https://doi.org/10.1007/s00216-009-3316-5

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