Skip to main content
SpringerLink
Log in

On-line determination of aliphatic amines in water using in-tube solid-phase microextraction-assisted derivatisation in in-valve mode for processing large sample volumes in LC

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

This paper describes a cost-effective procedure for the analysis of short-chain aliphatic amines in water samples using a solid-phase microextraction device. Analyte preconcentration and derivatisation were effected into a capillary column coated with 95% polydimethylsiloxane–5% polydiphenylsiloxane, which was used as the injection loop of a Rheodyne injection valve. The coating was previously loaded with the derivatisation reagent, 9-fluorenylmethyl chloroformate. A volume of 1 mL of samples was then drawn into the capillary column, and the extracted analytes were left to react on the capillary coating for 5 min. Next, the capillary column was cleaned by passing water. Finally, the injection valve was rotated, and the derivatives formed were dynamically desorbed and transferred to the analytical column into the mobile phase. Methylamine, ethylamine, propylamine, n-butylamine and n-pentylamine were selected as model compounds. Excellent sensitivity was achieved, being the limits of detection of 15–200 µg/L when using UV detection and of 0.1–0.4 µg/L by fluorescence.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Akyüz M, Ata S (2006) J Chromatogr A 1129:88–94

    Article  Google Scholar 

  2. You J, Zhao X, Suo Y, Wang H, Li Y, Sun J (2006) Anal Chim Acta 565:168–177

    Article  CAS  Google Scholar 

  3. Sahasrabuddhey B, Jain A, Verma KK (1999) The Analyst 124:1017–1021

    Article  CAS  Google Scholar 

  4. Verdú-Andrés J, Campíns-Falcó P, Herráez-Hernández R (2002) Chromatographia 55:129–134

    Article  Google Scholar 

  5. Cháfer-Pericás C, Campíns-Falcó P, Herráez-Hernández R (2004) J Chromatogr A 1023:27–31

    Article  Google Scholar 

  6. Herráez-Hernández R, Campíns-Falcó P, Sevillano-Cabeza A (1996) Anal Chem 68:734–739

    Article  Google Scholar 

  7. Pan L, Chong M, Pawliszyn J (1997) J Chromatogr A 773:249–260

    Article  CAS  Google Scholar 

  8. Lestremau F, Desauziers V, Fanlo JL (2001) The Analyst 126:1969–1973

    Article  CAS  Google Scholar 

  9. Cai L, Zhao Y, Gong S, Dong L, Wu C (2003) Chromatographia 58:615–621

    CAS  Google Scholar 

  10. Herráez-Hernández R, Cháfer-Pericás C, Campíns-Falcó P (2004) Anal Chim Acta 513:425–433

    Article  Google Scholar 

  11. Cháfer-Pericás C, Herráez-Hernández R, Campíns-Falcó P (2005) Talanta 66:1139–1145

    Article  Google Scholar 

  12. Herráez-Hernández R, Cháfer-Pericás C, Verdú-Andrés J, Campíns-Falcó P (2006) J Chromatogr A 1104:40–46

    Article  Google Scholar 

  13. Alpendurada M (2000) J Chromatogr A 889:3–14

    Article  CAS  Google Scholar 

  14. Ouyang G, Pawliszyn J (2006) Anal Bioanal Chem 386:1059–1073

    Article  CAS  Google Scholar 

  15. Lord HL (2007) J Chromatogr A 1152:2–13

    Article  CAS  Google Scholar 

  16. Olejniczak J, Staniewski J (2007) Anal Chim Acta 588:64–72

    Article  CAS  Google Scholar 

  17. Prieto-Blanco MC, Cháfer-Pericás C, López-Mahía P, Campíns-Falcó P (2008) J Chromatogr A 1188:118–123

    Article  CAS  Google Scholar 

  18. Campíns-Falcó P, Herráez-Hernández R, Sevillano-Cabeza A, Trümpler I (1997) Anal Chim Acta 344:125–136

    Article  Google Scholar 

  19. Lamba S, Pandit A, Sanghi SK, Gowri VS, Tiwari A, Baderia VK, Singh DK, Nigam P (2008) Anal Chim Acta 614:190–195

    Article  CAS  Google Scholar 

  20. Ábalos M, Bayona JM, Ventura F (1999) Anal Chem 71:3531–3537

    Article  Google Scholar 

  21. Liu X, Wang H, Liang S-C, Zhang H-S (2001) Anal Chim Acta 441:45–52

    Article  CAS  Google Scholar 

  22. Kaykhaii M, Nazari S, Chamsaz M (2005) Talanta 65:223–228

    Article  CAS  Google Scholar 

  23. Deng C, Li N, Wang L, Zhang X (2006) J Chromatogr A 1131:45–50

    Article  CAS  Google Scholar 

  24. Lambropoulou DA, Sakkas VS, Albanis TA (2002) J Chromatogr A 952:215–227

    Article  CAS  Google Scholar 

  25. EURACHEM/CITAC 2nd ed. (2000) Guide quantifying uncertainty in analytical measurement

Download references

Acknowledgments

The authors are grateful to the Spanish Ministerio de Ciencia y Tecnología (Project CTQ2007-65156/BQU) for financial support received. C. C.-P. is grateful to the Ministerio de Educación, Cultura y Deporte of Spain for pre-doctoral grant.

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain

    P. Campíns-Falcó, R. Herráez-Hernández, J. Verdú-Andrés & C. Cháfer-Pericás

Authors
  1. P. Campíns-Falcó
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Herráez-Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Verdú-Andrés
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Cháfer-Pericás
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. Campíns-Falcó.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Campíns-Falcó, P., Herráez-Hernández, R., Verdú-Andrés, J. et al. On-line determination of aliphatic amines in water using in-tube solid-phase microextraction-assisted derivatisation in in-valve mode for processing large sample volumes in LC. Anal Bioanal Chem 394, 557–565 (2009). https://doi.org/10.1007/s00216-009-2697-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-009-2697-9

Keywords

Search

Navigation