Skip to main content
SpringerLink
Log in

Determination of benzimidazole fungicides in water samples by on-line MISPE–HPLC

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

An analytical methodology based on an on-line sample enrichment of water samples by means of an imprinted polymer, and the separation of benzimidazole compounds within a C18 column by ion-pair reversed-phase liquid chromatography, has been developed. The molecularly imprinted polymer has been synthesized by precipitation polymerization using thiabendazole as template molecule, methacrylic acid as functional monomer, and divinylbenzene as cross-linker. Initial experiments carried out by solid-phase extraction on cartridges demonstrated a clear imprint effect for thiabendazole, as well as the ability of the imprinted polymer to selectively rebind several benzimidazole compounds. The developed methodology has been applied to the quantification of thiabendazole, carbendazim, and benomyl in river, tap, and well water samples within a single analytical run at concentration levels below the legislated maximum concentration levels. In this sense, detection limits of 2.3–5.7 ng·L−1 have been obtained for the analysis of benzimidazole fungicides in different water matrices. Recoveries obtained for the determination of benzimidazole fungicides in spiked samples ranged from 87% to 95%, with RSD below 5% in all cases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Danaher M, De Ruyck H, Crooks SRH, Dowling G, O’Keeffe M (2007) J Chromatogr B 845:1

    Article  CAS  Google Scholar 

  2. European Parliament (2006) 2006/11/CE Directive

  3. Jeannot R, Sabik H, Sauvard E, Gessin E (2000) J Chromatogr A 879:51

    Article  CAS  Google Scholar 

  4. Zamora DP, Vidal JLM, Galera MM, Frenich AG, Gonzalez JLL, Arahal MR (2003) Talanta 60:335

    Article  Google Scholar 

  5. Frenich AG, Zamora DP, Vidal JLM, Galera MM (2003) Anal Chim Acta 477:211

    Article  Google Scholar 

  6. Hogendoorn EA, Westhuis K, Dijkman E, Eusingkweld EAG, Chauvaskul P, Biadul P, Baumann RA, Cornelese AA, van der Linden MA (2000) Int J Environ Anal Chem 78:67

    Article  CAS  Google Scholar 

  7. JunkerBuchheit A, Witzenbacher M (1996) J Chromatogr A 737:67

    Article  CAS  Google Scholar 

  8. Shimamura Y, Tomiyama N, Murakoshi M, Kobayashi M, Matano O (1998) J Pest Sci 23:241

    CAS  Google Scholar 

  9. Halko R, Sanz CP, Ferrera ZS, Rodriguez JJS (2004) Chromatographia 60:151

    Article  CAS  Google Scholar 

  10. Moral A, Sicilia MD, Rubio S, Perez-Bendito D (2006) Anal Chim Acta 569:132

    Article  CAS  Google Scholar 

  11. de Prada AGV, Loaiza OA, Serra B, Morales D, Martinez-Ruiz P, Reviejo AJ, Pingarron JM (2007) Anal Bioanal Chem 388:227

    Article  Google Scholar 

  12. Turiel E, Tadeo JL, Cormack PAG, Martin-Esteban A (2005) Analyst 130:1601

    Article  CAS  Google Scholar 

  13. Cacho C, Schweitz L, Turiel E, Perez-Conde C (2008) J Chromatogr A 1179:216

    Article  CAS  Google Scholar 

  14. Cacho C, Turiel E, Perez-Conde C (2009) Talanta (in press)

  15. Caro E, Marce EM, Cormack PAG, Sherrington DC, Borrull F (2003) J Chromatogr A 995:233

    Article  CAS  Google Scholar 

  16. Benito Peña E, Urraca JL, Sellergren B, Moreno-Bondi MC (2008) J Chromatogr A 1208:62

    Article  Google Scholar 

  17. Yin J, Wang S, Yang G, Yang G, Chen Y (2006) J Chromatogr B 844:142

    Article  CAS  Google Scholar 

  18. Mallat E, Barcelo D, Tauler R (1997) Chromatographia 46:342

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to thank CONACyT (46558-Y, Mexico), DGCYT (CTQ2005-02281), Comunidad de Madrid (S-0505/AGR/0312), and Universidad Complutense de Madrid for financial support.

Author information

Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Chemistry, Universidad Nacional Autónoma de México, 04510, México DF, Mexico

    O. Zamora & L. E. Vera-Avila

  2. Analytical Chemistry Department, Faculty of Chemistry, Universidad Complutense de Madrid, 28040, Madrid, Spain

    E. E. Paniagua, C. Cacho & C. Perez-Conde

Authors
  1. O. Zamora
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. E. Paniagua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Cacho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. E. Vera-Avila
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Perez-Conde
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Perez-Conde.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zamora, O., Paniagua, E.E., Cacho, C. et al. Determination of benzimidazole fungicides in water samples by on-line MISPE–HPLC. Anal Bioanal Chem 393, 1745–1753 (2009). https://doi.org/10.1007/s00216-009-2631-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-009-2631-1

Keywords

Search

Navigation