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Fluorescence properties of selected benzo[c]phenantridine alkaloids and studies of their interaction with CT DNA

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Abstract

The spectral, especially fluorescence properties, of seven selected quaternary benzo[c]phenantridine alkaloids (sanguinarine, chelerythrine, chelirubine, sanguirubine, chelilutine, sanguilutine, and macarpine) were studied in presence and in absence of double-stranded DNA. This study has proved dramatic differences in fluorescence emission of all studied alkaloids in presence of calf thymus DNA in comparison to fluorescence of free alkaloids. The most remarkable are changes in emission spectra of macarpine, chelirubine, and sanguirubine. Association constants (logK) for interaction of all studied alkaloids with CT DNA were calculated.

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Acknowledgments

This work was supported by projects CSF 525/08/0819 and LC06035 BIO-ANAL-MED.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137, Brno, Czech Republic

    Jana Urbanová, Přemysl Lubal & Petr Táborský

  2. Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 61137, Brno, Czech Republic

    Iva Slaninová

  3. Department of Chemistry and Biochemistry, Faculty of Medicine, Masaryk University, Kamenice 2, 61137, Brno, Czech Republic

    Eva Táborská

Authors
  1. Jana Urbanová
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  2. Přemysl Lubal
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  3. Iva Slaninová
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  4. Eva Táborská
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  5. Petr Táborský
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Corresponding author

Correspondence to Petr Táborský.

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Urbanová, J., Lubal, P., Slaninová, I. et al. Fluorescence properties of selected benzo[c]phenantridine alkaloids and studies of their interaction with CT DNA. Anal Bioanal Chem 394, 997–1002 (2009). https://doi.org/10.1007/s00216-009-2601-7

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  • DOI: https://doi.org/10.1007/s00216-009-2601-7

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