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Chiral ligand-exchange chromatography of amino acids using porous graphitic carbon coated with a dinaphthyl derivative of neamine

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Abstract

In this paper, we describe the preparation and the evaluation of a porous graphitic carbon (PGC) column coated with a new dinaphthyl derivative of neamine for chiral ligand-exchange (LE) chromatography. It was shown that the graphitic surface/dinaphthyl anchor system efficiently (1.15 μmol/m2) and stably (three months of intensive use) adsorbs the neamine template onto the chromatographic support. The resulting coated PGC stationary phase showed appreciable LE-based enantioselective properties towards several native amino acids.

Chromatographic separation of methionine enantiomers using a dinaphtyl neamine-based ligand-exchange chiral stationary phase

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Authors and Affiliations

  1. Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Grenoble I (Joseph Fourier), UFR de Pharmacie, 38041, Grenoble cedex 9, France

    Mustapha Zaher, Corinne Ravelet, Isabelle Baussanne, Anne Ravel, Catherine Grosset, Jean-Luc Décout & Eric Peyrin

Authors
  1. Mustapha Zaher
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  2. Corinne Ravelet
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  3. Isabelle Baussanne
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  4. Anne Ravel
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  5. Catherine Grosset
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  6. Jean-Luc Décout
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  7. Eric Peyrin
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Correspondence to Eric Peyrin.

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Zaher, M., Ravelet, C., Baussanne, I. et al. Chiral ligand-exchange chromatography of amino acids using porous graphitic carbon coated with a dinaphthyl derivative of neamine. Anal Bioanal Chem 393, 655–660 (2009). https://doi.org/10.1007/s00216-008-2488-8

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  • DOI: https://doi.org/10.1007/s00216-008-2488-8

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