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Implementation of terbium-sensitized luminescence in sequential-injection analysis for automatic analysis of orbifloxacin

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Abstract

Orbifloxacin (ORBI) is a third-generation fluoroquinolone developed exclusively for use in veterinary medicine, mainly in companion animals. This antimicrobial agent has bactericidal activity against numerous Gram-negative and Gram-positive bacteria. A few chromatographic methods for its analysis have been described in the scientific literature. Here, coupling of sequential-injection analysis and solid-phase spectroscopy is described in order to develop, for the first time, a terbium-sensitized luminescent optosensor for analysis of ORBI. The cationic resin Sephadex-CM C-25 was used as solid support and measurements were made at 275/545 nm. The system had a linear dynamic range of 10–150 ng mL−1, with a detection limit of 3.3 ng mL−1 and an R.S.D. below 3% (n = 10). The analyte was satisfactorily determined in veterinary drugs and dog and horse urine.

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Acknowledgements

The authors are grateful to the “Consejería de Innovación, Ciencia y Empresa (Junta de Andalucía)”, Project P07-FQM-02673, for financial support. E.J.L.M. acknowledges a program scholarship from Junta of Andalucía.

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Authors and Affiliations

  1. Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, 23071, Jaén, Spain

    E. J. Llorent-Martínez, P. Ortega-Barrales, A. Molina-Díaz & A. Ruiz-Medina

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  1. E. J. Llorent-Martínez
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  2. P. Ortega-Barrales
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  3. A. Molina-Díaz
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  4. A. Ruiz-Medina
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Correspondence to A. Ruiz-Medina.

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Llorent-Martínez, E.J., Ortega-Barrales, P., Molina-Díaz, A. et al. Implementation of terbium-sensitized luminescence in sequential-injection analysis for automatic analysis of orbifloxacin. Anal Bioanal Chem 392, 1397–1403 (2008). https://doi.org/10.1007/s00216-008-2434-9

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  • DOI: https://doi.org/10.1007/s00216-008-2434-9

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