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Short-end injection capillary electrophoresis for quantification of plasma chondroitin sulfate isomer disaccharides

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Abstract

We describe a new ultra-rapid capillary electrophoresis method with UV detection for analysis of the disaccharides obtained after enzymatic depolymerization of plasma chondroitin sulfates. The free reducing groups of the released carbohydrate molecules are derivatized with 2-aminoacridone by reductive amination in the presence of cyanoborohydride. The fluorotagged products can be separated by short-end injection capillary electrophoresis in a capillary with an effective length of 10.2 cm. The migration times of Δdi-0S and Δdi-4S were 0.95 and 1.81 min, respectively. We compared the proposed method with UV detection to a reference CE-LIF assay by measuring plasma chondroitin sulfate in 94 subjects. The described assay for total plasma CS measurement may, owing to the high throughput and the fast analytical times, be a good tool for routine studies both in research and in clinical applications.

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Abbreviations

GAG:

Glycosaminoglycan

CS:

Chondroitin sulfate

DS:

Dermatan sulphate

AMAC:

2-Aminoacridone

CE:

Capillary electrophoresis

LIF:

Laser-induced fluorescence

FACE:

Fluorophore-assisted carbohydrate electrophoresis

Δdi-0S:

2-Acetamido-2-deoxy-3-O-(4-deoxy-a-l-threo-hex-4-enepyranosyluronic acid)-d-galactose

Δdi-4S:

2-Acetamido-2-deoxy-3-O-(4-deoxy-a-l-threo-hex-4-enepyranosyluronic acid)-d-galactose 4-sulfate

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Acknowledgements

This study was supported by the “Fondazione Banco di Sardegna – Sassari – Italy” and by the “Ministero dell’Università e della Ricerca” Italy. Manuscript language revision by Mrs Maria Antonietta Meloni is greatly appreciated.

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Authors and Affiliations

  1. Department of Biomedical Sciences, Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro, 43/B-07100, Sassari, Italy

    Angelo Zinellu, Salvatore Sotgia, Maria Franca Usai, Luca Deiana & Ciriaco Carru

  2. National Laboratory of the National Institute of Biostructures and Biosystems, Osilo, Italy

    Angelo Zinellu & Ciriaco Carru

  3. Department of Medicinal and Toxicological Chemistry, University of Sassari, Sassari, Italy

    Salvatore Sotgia

  4. Department of Physiological, Biochemical, and Cellular Science, University of Sassari, Sassari, Italy

    Elisabetta Zinellu, Antonio J. Lepedda & Marilena Formato

Authors
  1. Angelo Zinellu
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  2. Salvatore Sotgia
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  3. Maria Franca Usai
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  4. Elisabetta Zinellu
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  5. Antonio J. Lepedda
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  6. Luca Deiana
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  7. Marilena Formato
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  8. Ciriaco Carru
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Corresponding authors

Correspondence to Angelo Zinellu or Ciriaco Carru.

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Zinellu, A., Sotgia, S., Usai, M.F. et al. Short-end injection capillary electrophoresis for quantification of plasma chondroitin sulfate isomer disaccharides. Anal Bioanal Chem 391, 2865–2868 (2008). https://doi.org/10.1007/s00216-008-2202-x

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  • DOI: https://doi.org/10.1007/s00216-008-2202-x

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