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Validation of a screening method for rapid control of macrocyclic lactone mycotoxins in maize flour samples

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Abstract

A procedure for the analytical validation of a rapid supercritical fluid extraction amperometric screening method for controlling macrocyclic lactone mycotoxins in maize flour samples has been developed. The limit established by European legislation (0.2 mg kg−1), in reference to zearalenone (ZON) mycotoxin, was taken as the reference threshold to validate the proposed method. Natural ZON metabolites were also included in this study to characterize the final screening method. The objective was the reliable classification of samples as positive or negative samples. The cut-off level was fixed at a global concentration of mycotoxins of 0.17 mg kg−1. An expanded unreliability zone between 0.16 and 0.23 mg kg−1 characterized the screening method for classifying the samples. A set of 30 samples was used for the final demonstration of the reliability and usefulness of the method.

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Acknowledgements

The Spanish Ministerio de Educación y Ciencia and JJCC Castilla-La Mancha are gratefully acknowledged for funding this work with Grants CTQ2007–61830 and PCC08–015–0722. The support given through a “Juan de la Cierva” research contract for M. Zougagh is also acknowledged.

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Authors and Affiliations

  1. Department of Analytical Chemistry and Food Technology, Faculty of Chemistry, University of Castilla–La Mancha, Av. Camilo José Cela s/n, 13004, Ciudad Real, Spain

    Mohammed Zougagh, Helena Téllez & Angel Ríos

  2. Department of Analytical Chemistry and Instrumental Analysis, Faculty of Science, Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Alberto Sánchez & Manuel Chicharro

Authors
  1. Mohammed Zougagh
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  2. Helena Téllez
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  3. Alberto Sánchez
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  4. Manuel Chicharro
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  5. Angel Ríos
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Correspondence to Angel Ríos.

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Zougagh, M., Téllez, H., Sánchez, A. et al. Validation of a screening method for rapid control of macrocyclic lactone mycotoxins in maize flour samples. Anal Bioanal Chem 391, 709–714 (2008). https://doi.org/10.1007/s00216-008-2047-3

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  • DOI: https://doi.org/10.1007/s00216-008-2047-3

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