Abstract
Fusarium species are one of the common pathogenic fungi of vegetable diseases. Some Fusarium species produce toxic secondary metabolites, which have potential health risks for humans and animals. In the study, an ultra-performance liquid chromatography tandem mass spectrometry method (UPLC-MS/MS) was developed for the analysis of 13 emerging and traditional Fusarium toxins in pepper, tomato, cucumber, and potato. Samples were extracted by a modified quick, easy, cheap, effective, rugged, and safe method. Dispersive solid-phase extraction was employed for the removal of matrix interferences from the crude extracts of various vegetables. Matrix effect was compensated using matrix-matched calibration curves. The method was fully validated according to the European Commission Decision 2002/657/EC. For all the targeted analytes, good linearity (R2 ≥ 0.9823) was obtained. Recoveries were in the range of 62 and 114% in pepper, tomato, cucumber, and potato, with intra-day relative standard deviations lower than 14.3%. Quantitation limits of the developed method were between 0.25 and 20 μg/kg in four sample matrices. The method was successfully employed for the analysis of Fusarium toxins in vegetable samples.
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Funding
The study was funded by the National Natural Science Foundation of China (31401598, 31602124), Shanghai Agriculture Commission Basic Research Program (2014 No. 7-3-7), Science and Technology Commission of Shanghai Municipality Research Program (16391903800), Natural Science Foundation of Shanghai (13ZR1440500), and National Major Project for Agro-product Quality and Safety Risk Assessment (GJFP201700102).
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Zhiyong Zhao declares that he has no conflict of interest. Jianhua Wang declares that he has no conflict of interest. Xianli Yang declares that he has no conflict of interest. Changyan Zhou declares that she has no conflict of interest. Xiaoyan Zhao declares that she has no conflict of interest. Bing Bai declares that he has no conflict of interest. Lei Chen declares that he has no conflict of interest.
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Zhao, Z., Yang, X., Zhao, X. et al. Method Development and Validation for the Analysis of Emerging and Traditional Fusarium Mycotoxins in Pepper, Potato, Tomato, and Cucumber by UPLC-MS/MS. Food Anal. Methods 11, 1780–1788 (2018). https://doi.org/10.1007/s12161-018-1180-7
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DOI: https://doi.org/10.1007/s12161-018-1180-7