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Toward hybridization assays without PCR using universal nanoamplicons

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Abstract

An innovative scheme for signal amplification using random tetramer-modified gold nanoparticles, termed “nanoamplicons,” has been developed for hybridization assay without PCR. Large numbers of nanoamplicons could be integrated onto one target, providing much greater amplification than the larger nanoparticles usually adopted. Using M13mp18 single-strand DNA as a target, this concept is shown to be a feasible approach to detecting 0.17 amol L−1 DNA without target amplification, based on microgravimetric detection of the adsorption of the probe–target–nanoamplicons complex via thiol–gold binding. To our knowledge, this method has a sensitivity that is close to that of PCR and superior to those of nanoparticle-based methods reported previously. Additionally, this novel nanoamplicon could be prepared in the same way and used for all diagnostic tests; such universality would make the nanoamplicons highly advantageous for the generalization and standardization of bioassays, and when applying this new technology in clinical laboratories.

A novel signal amplification method for DNA detection with subattomolarsensitivity has been developed using random tetramer-modified gold nanoparticlesas nanoamplicons, which are easily prepared with high uniformity and can be universally adaptedto any sequences

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Acknowledgments

This work was partially supported by the National Ministry of Science and Technology of the People’s Republic of China under Contracts 03C26215100252.

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Authors and Affiliations

  1. Micro-System Research Center, College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, People’s Republic of China

    Z.-H. Mo

  2. College of Environmental and Biological Engineering, Chongqing University of Technology and Business, Chongqing, 400067, People’s Republic of China

    X.-L. Wei

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  1. Z.-H. Mo
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  2. X.-L. Wei
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Correspondence to Z.-H. Mo.

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Mo, ZH., Wei, XL. Toward hybridization assays without PCR using universal nanoamplicons. Anal Bioanal Chem 386, 2219–2223 (2006). https://doi.org/10.1007/s00216-006-0877-4

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  • DOI: https://doi.org/10.1007/s00216-006-0877-4

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