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Microgravimetric flow analysis of nucleic acid based on adsorption of nanoparticle-bioconjugate

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Abstract

A novel nanoparticle-bioconjugate has been prepared by specific hybridization of the target with complementary thiol-labeled and nanoparticle-labeled probes. The rapid adsorption of the nanoparticle-bioconjugate onto a gold surface via a thiol-gold reaction was monitored in real-time using a quartz crystal microbalance, and used to perform microgravimetric flow analysis of nucleic acid for the first time. This innovative assay is highly reproducible and sensitive, and shows great promise for clinical applications.

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Acknowledgements

This work was supported by the National Ministry of Science and Technology of the People’s Republic of China under Contract 03C26215100252. We also thank P. Jin for helpful suggestions.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, PR China

    Z.-H. Mo, Y.-L. Liang & H.-L. Wang

  2. Chengdu Enwei Pharmaceutical Co. Ltd., Chengdu, 610041, Sichuan, PR China

    F.-W. Liu & Y.-X. Xue

  3. Chongqing University of Medical Science, Chongqing, 400016, PR China

    H.-L. Wang

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  1. Z.-H. Mo
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  2. Y.-L. Liang
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  3. H.-L. Wang
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  4. F.-W. Liu
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  5. Y.-X. Xue
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Correspondence to Z.-H. Mo.

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Mo, ZH., Liang, YL., Wang, HL. et al. Microgravimetric flow analysis of nucleic acid based on adsorption of nanoparticle-bioconjugate. Anal Bioanal Chem 382, 996–1000 (2005). https://doi.org/10.1007/s00216-005-3231-3

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  • DOI: https://doi.org/10.1007/s00216-005-3231-3

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