Skip to main content
SpringerLink
Log in

Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)

  • Special Issue Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

In this work four different commercially available enzyme-linked immunosorbent assays (ELISA) (from Japan EnviroChemicals, Ltd., Tokyo, Japan) were evaluated in terms of performance for the rapid screening of estrogens in different water matrices, including natural and spiked samples from urban wastewater, river water and ground water. All four test kits are based on monoclonal antibodies. The compounds detected by these immunoassays are (1) 17-β-estradiol, (2) estrone, (3) 17-α-ethynyl estradiol and (4) estrogens in general, with high recognition properties for 17-β-estradiol, estrone and estriol. Standards were prepared in water containing 10% (v/v) methanol. The IC 50 (corresponding to the 50% of the effective concentration) values, the dynamic ranges, and the limits of detection of the ELISA kits were 0.060–0.304 μg/L, 0.05–5 μg/L and 0.05 μg/L, respectively. All samples were extracted by solid-phase extraction (SPE) beforehand, and the evaluation was carried out by comparing the results obtained by ELISA with those obtained by HPLC–MS/MS using a triple quadrupole (QqQ) instrument. In addition, two different solid-phase extraction procedures were carried out and compared. Except for moderate overestimation in the results observed with the ELISA kits in the analysis of complex wastewater samples, the results obtained using all of the tested techniques were generally in very good agreement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3a–b
Fig. 4a–b
Fig. 5a–h

Similar content being viewed by others

References

  1. Davis DL, Axelrod D, Osborne MP, Telang N (1997) Sci Med 4:56

    Google Scholar 

  2. Bhatt RV (2000) Int J Gynecol Obstet 70:69

    Article  CAS  Google Scholar 

  3. Boccardo F, Puntoni M, Guglielmini P, Rubagotti A (2006) Clin Chim Acta 365:58

    Google Scholar 

  4. Andersen AG, Jensen TK, Carlsen E, Jorgensen N, Andersson AM, Krarup T, Keiding N, Skakkebaek NE (2000) Hum Reprod 15:366

    Article  CAS  Google Scholar 

  5. Massaad C, Entezami F, Massade L, Benahmed M, Olivennes F, Barouki R, Hamamah S (2002) J Obstet Gynecol Reprod Biol 100:127

    Article  CAS  Google Scholar 

  6. Solé M, Porte C, Barceló D (2001) Trends Anal Chem 20:518

    Google Scholar 

  7. Rodgers-Gray TP, Jobling S, Kelly C, Morris S, Brighty G, Waldock MJ, Sumpter JP, Tyler CR (2001) Environ Sci Technol 35:462

    Article  CAS  Google Scholar 

  8. Metzler M (2001) The handbook of environmental chemistry, vol 3. Part L. Endocrine disruptors, Part I. Springer, Berlin Heidelberg New York

  9. Ian S, Fraser RP, Jansen RA, Lobo MI (1998) Estrogens and progestogens in clinical practice. Churchill Livingstone, Whitehead

  10. Ternes TA, Kreckel P, Mueller (1999) J Sci Total Environ 225:91

    Article  CAS  Google Scholar 

  11. Baronti C, Curini G, Di Corcia A, Gentili A, Samperi R (2000) Environ Sci Technol 34:5059

    Article  CAS  Google Scholar 

  12. Ternes TA, Andersen H, Gilberg D, Bonerz M (2002) Anal Chem 74:3498

    Article  CAS  Google Scholar 

  13. Peck M, Gibson RW, Kortenkamp A, Hill EM (2004) Environ Toxicol Chem 23:945

    Article  CAS  Google Scholar 

  14. Williams RJ, Johnson AC, Smith JJL, Kanda R (2003) Environ Sci Technol 37:1744

    Article  CAS  Google Scholar 

  15. López de Alda MJ, Barceló D (2000) J Chromatogr A 892:391

    Article  Google Scholar 

  16. López de Alda MJ, Barceló D (2001) J Chromatogr A 911:203

    Article  Google Scholar 

  17. Díaz Cruz MS, López de Alda MJ, López R, Barceló D (2003) J Mass Spectrom 38:917

    Article  Google Scholar 

  18. National Water Quality Monitoring Council (NWQMC) (2004) National Monitoring Conference Building and Sustaining Successful Monitoring Programs. Chattanooga, TN, USA

  19. Rodríguez-Mozaz S, López de Alda MJ, Barceló D (2004) J Chromatogr A 1045:85

    Article  Google Scholar 

  20. Rodríguez-Mozaz S, López de Alda MJ, Barceló D (2004) Anal Chem 76:6998

    Article  Google Scholar 

  21. Laganà A, Bacaloni A, De Leva I, Fabreri A, Fago G, Marino A (2004) Anal Chim Acta 501:79–88

    Article  Google Scholar 

  22. Anderson H, Seigrist H, Halling-Soreson B, Ternes TA (2003) Environ Sci Technol 37:4021–4026

    Article  Google Scholar 

  23. EC Health and Consumer Protection Directorate SANCO (2005) General Directorate D: Food Safety: production and distribution chain. SANCO/10439/2005 revision 8, 6 July 2005. European Commission, Brussels

Download references

Acknowledgements

This work was supported by the EU through the projects SWIFT-WFD (SSPI-CT-2003-502492) and MODELKEY (SSPI-CT-2003-511237-2), and by the Ministerio de Ciencia y Tecnología through the projects CTM2005-24255-E and EVITA (CTM2004-06265-C03-01/TECNO). Marinella Farré thanks the support from the Ministerio de Educación y Ciencia through the Juan de la Cierva program.

This article reflects only the authors views and the EU is not liable for any use that may be made of the information contained therein.

Author information

Authors and Affiliations

  1. Department of Environmental Chemistry, IIQAB-CSIC, C/ Jordi Girona 18-26, 08034, Barcelona, Spain

    Marinella Farré, Rikke Brix, Marina Kuster, María J. López de Alda & Damià Barceló

  2. Sensors and Biosensors Group, Department of Analytical Chemistry, UAB, Bellaterra, Cerdanyola del Valles, 08193, Spain

    Marinella Farré

  3. Abraxis, 54 Steamwhistle Drive, Warminster, PA, 18974, USA

    Fernando Rubio

  4. Japan EnviroChemicals, Ltd., 2-17-85 Juso-Honmachi Yodogawa-ku, Osaka, 532-0024, Japan

    Yasuhiro Goda

Authors
  1. Marinella Farré
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rikke Brix
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marina Kuster
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fernando Rubio
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yasuhiro Goda
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. María J. López de Alda
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Damià Barceló
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Damià Barceló.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Farré, M., Brix, R., Kuster, M. et al. Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ). Anal Bioanal Chem 385, 1001–1011 (2006). https://doi.org/10.1007/s00216-006-0562-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-006-0562-7

Keywords

Search

Navigation