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Highly sensitive spectrofluorimetric determination of trace amounts of lecithin

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Abstract

A new spectrofluorimetric method was developed for the determination of trace amounts of lecithin using the ciprofloxacin (CIP)–terbium (Tb3+) ion complex as a fluorescent probe. In a buffer solution at pH=5.60, lecithin can remarkably reduce the fluorescence intensity of the CIP–Tb3+ complex at λ=545 nm. The reduced fluorescence intensity of the Tb3+ ion is proportional to the concentration of lecithin. Optimum conditions for the determination of lecithin were also investigated. The linear range and detection limit for the determination of lecithin were 1.0×10−6–3.0×10−5 mol L−1 and 3.44×10−7 mol L−1, respectively. This method is simple, practical, and relatively free of interference from coexisting substances. Furthermore, it has been successfully applied to assess lecithin in serum samples.

 

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References

  1. Campanella L, Pacifici F, Sammartino MP, Tomassetti M (1998) Bioelectrochem Bioenerg 47:25–38

    Article  CAS  Google Scholar 

  2. Campanella L, Magri AL, Sorbo A, Tomassetti M (2002) J Pharm Biomed Anal 29:1135–1148

    Article  PubMed  CAS  Google Scholar 

  3. Baenholzd Y (1980) Biochime Biophys Acta 604:129

    Article  Google Scholar 

  4. Yie YW, Li JZ, Wang YC (1989) Clinical experimental diagnostics. People Sanitation Press, Beijing, p 674

    Google Scholar 

  5. Guo Q, Xu GY, Chang LW (1998) Chin J Anal Chem vol 81–84

  6. Hanras C, Perrin JL (1991) J AOCS 68:302–309

    Google Scholar 

  7. Hradec J, Dufek P (1997) J Chromatography B 703:259–262

    Article  CAS  Google Scholar 

  8. Ceve G, Paltauf F (1995) (eds) Phospholipids: characterization, metabolism, and novel biological applications. AOCS

  9. Faju H, Chongqiu J (2005) Anal Lett 38(2):281–289

    Article  Google Scholar 

  10. Yusheng W, Lin F, Chongqiu J (2005) Spectrochim Acta A 61:2909–2914

    Article  CAS  Google Scholar 

  11. Richardson FS (1982) Chem Rev 82:541–552

    Article  Google Scholar 

  12. Yuan JL, Wang GL, Majima K, Matsumoto K (2001) Anal Chem 73:1869–1876

    Article  PubMed  CAS  Google Scholar 

  13. Xing da Zeng (2001) Applied clinic handbook. Guangdong Science and Technology, China

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Chemistry, Shandong Normal University, Jinan, 250014, China

    Weiwei Bian & Chongqiu Jiang

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  1. Weiwei Bian
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  2. Chongqiu Jiang
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Correspondence to Chongqiu Jiang.

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Bian, W., Jiang, C. Highly sensitive spectrofluorimetric determination of trace amounts of lecithin. Anal Bioanal Chem 385, 861–865 (2006). https://doi.org/10.1007/s00216-006-0488-0

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  • DOI: https://doi.org/10.1007/s00216-006-0488-0

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