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Arsenic speciation in xylem sap of cucumber (Cucumis sativus L.)

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Abstract

Flow injection analysis (FIA) and high-performance liquid chromatography double-focusing sector field inductively coupled plasma mass spectrometry (HPLC-DF-ICP-MS) were used for total arsenic determination and arsenic speciation of xylem sap of cucumber plants (Cucumis sativus L.) grown in hydroponics containing 2 μmol dm−3 arsenate or arsenite, respectively. Arsenite [As(III)], arsenate [As(V)] and dimethylarsinic acid (DMA) were identified in the sap of the plants. Arsenite was the predominant arsenic species in the xylem saps regardless of the type of arsenic treatment, and the following concentration order was determined: As(III) > As(V) > DMA. The amount of total As, calculated taking into consideration the mass of xylem sap collected, was almost equal for both treatments. Arsenite was taken up more easily by cucumber than arsenate. Partial oxidation of arsenite to arsenate (<10% in 48 h) was observed in the case of arsenite-containing nutrient solutions, which may explain the detection of arsenate in the saps of plants treated with arsenite.

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Acknowledgements

The authors express their gratitude to Prof. P. Fodor (University of Horticulture and Food Industry, Budapest) and to Prof. S. Caroli (Istituto Superiore di Sanità, Rome) for the kind donation of MMA and of organoarsenicals, respectively.

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Authors and Affiliations

  1. Joint Research Group of Environmental Chemistry of the Hungarian Academy of Sciences and L. Eötvös University, PO Box 32, 1518, Budapest, Hungary

    Victor G. Mihucz & Gyula Záray

  2. Hungarian Satellite Centre of Trace Elements Institute to UNESCO, PO Box 32, 1518, Budapest, Hungary

    Victor G. Mihucz, Enikő Tatár & Gyula Záray

  3. Department of Inorganic and Analytical Chemistry, L. Eötvös University, PO Box 32, 1518, Budapest, Hungary

    Enikő Tatár, István Virág & Gyula Záray

  4. Department of Plant Physiology, L. Eötvös University, PO Box 120, 1518, Budapest, Hungary

    Edit Cseh & Ferenc Fodor

Authors
  1. Victor G. Mihucz
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  2. Enikő Tatár
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  3. István Virág
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  4. Edit Cseh
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  5. Ferenc Fodor
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  6. Gyula Záray
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Correspondence to Gyula Záray.

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Mihucz, V.G., Tatár, E., Virág, I. et al. Arsenic speciation in xylem sap of cucumber (Cucumis sativus L.). Anal Bioanal Chem 383, 461–466 (2005). https://doi.org/10.1007/s00216-005-3325-y

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  • DOI: https://doi.org/10.1007/s00216-005-3325-y

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