Abstract
A simple, sensitive and specific flow-injection spectrofluorimetric method has been developed for the determination of folic acid in pharmaceuticals. The method is based on use of a lead dioxide solid-phase reactor for on-line oxidation of folic acid into a strongly fluorescent compound with a maximum excitation wavelength of 281 nm and an emission wavelength of 450 nm. Under optimum conditions the fluorescence intensity of oxidation product is proportional to the concentration of folic acid over the range 0.008–2.5 μg mL−1. The detection limit is 0.0001 μg mL−1, the relative standard deviation is 0.85% for 11 replicate determinations of 0.05 μg mL−1 folic acid, and the sample throughput is 20 h−1. In combination with an on-line filter and dilution, an automated drug-dissolution system was established. The proposed method has been successfully applied to the determination of folic acid in pharmaceutical preparations and dissolution testing.
Similar content being viewed by others
References
Lucock M (2000) Molecular genetics and metabolism 71:121–138
Buhl F, Hachula U (1991) Chem Anal (Warsaw) 36:27–34
Aberasturi FJ, Jimenez AI, Arias JJ, Jimenez F (2002) Anal Lett 35:1677–1691
Andrisano V, Bartolini M, Bertucci C, Cavrini V, Lnppi B, Cerchiara T (2003) J Pharm Biomed Anal 32:983–989
Le Gall AC, Van den Berg CMG (1993) Anal Chim Acta 282:459–470
Szczepaniak W, Ren M (1994) Electroanalysis 6:505–507
Ichinose N, Tsuneyoshi T, Kato M, Suzuki T, Ikeda S (1993) Fresenius J Anal Chem 346:841–846
Akhtar MJ, Khan MA, Ahmad I (1997) J Pharm Biomed Anal 16:95–99
Osseyi ES, Wehling RL, Albrecht JA (1998) J Chromatogr A 826:235–240
Stokes P, Webb K (1999) J Chromatogr A 864:59–67
Garbis SD, Melse-Boonstra A, West CE, Van Breemen RB (2001) Anal Chem 73:5358–5364
Alwarthan AA (1994) Anal Sci 10:919–922
Sun CY, Zhao HC, Ou YJ (2000) Chin J Anal Lab 19:60–62
Nie F, He YH, Lu JR (2000) Microchem J 65:319–323
Lapa RAS, Lima JLFC, Reis BF, Santos JLM, Zagatto EAG (1997) Anal Chim Acta 351:223–228
Huang CB, Chen HW, He QH (2003) Chin J Anal Chem 31:229–231
Liu X, Huang HG (2000) Chin J Anal Chem 28:1406–1049
Liu X, Huang HG (2002) Chin J Anal Chem 30:1018
The Pharmacopoeia of The People’ s Republic of China (1995) Chem Ind Press 2:75
Dow AD, Finlay G, Bloomfield MS (1999) J Pharm Biomed Anal 19:549–554
Fang ZL (1999) Anal Chim Acta 400:233–247
Solich P, Polydorou CK, Koupparis MA, Efstathiou CE (2001) Anal Chim Acta 438:131–136
Legnerová Z, Huclová J, Thun R, Solich P (2004) J Pharm Biomed Anal 34:115–121
Gálvez AM, García Mateo JVG, Calatayud JM (2002) J Pharm Biomed Anal 27:1027–1036
Acknowledgements
This project was supported by the National Natural Science Foundation of China (No. 20175012) and the Key Project of Chinese Ministry of Education (No. 01156).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, ZQ., Tang, Y. Solid-phase reactor flow-injection on-line oxidizing spectrofluorimetry for determination and dissolution studies of folic acid. Anal Bioanal Chem 381, 932–936 (2005). https://doi.org/10.1007/s00216-004-2961-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-004-2961-y