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Application of combined approach to analyze the constituents of essential oil from Dong quai

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Abstract

A combined approach of sub-window factor analysis and spectral correlative chromatography has been employed to analyze the constituents of essential oils of Dong quai. Essential oils are the main pharmacological active individuals of Dong quai. Some constituents in the main root of Dong quai have been identified by GC-MS with the help of sub-window factor analysis resolving two-dimensional original data into mass spectra and chromatograms. Correlative constituents in another part of the root fiber have been recognized by spectral correlative chromatography. Seventy six of 97 separated constituents in the essential oil of main root were identified and quantified, accounting for about 91.36% of the total content. Sixty seven correlative components in the essential oil of root fiber were recognized. The result proves that the combined approach is powerful enough for the analysis of complex herbal samples.

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Acknowledgements

This work was financially supported by the National Nature Foundation Committee of P. R. China (no. 20175036 and no. 20235020). The authors gratefully acknowledge the experimental assistance of associate professor You-Qun Song and senior engineer Hui Cui (State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University) for GC-MS analysis.

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Authors and Affiliations

  1. Research Center of Modernization of Chinese Herbal Medicines, College of Chemistry and Chemical Engineering, Central South University, 410083, Changsha, P. R. China

    Lan-Fang Huang, Bo-Yan Li, Yi-Zeng Liang, Fang-Qiu Guo & Ya-Li Wang

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  1. Lan-Fang Huang
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  2. Bo-Yan Li
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  3. Yi-Zeng Liang
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  4. Fang-Qiu Guo
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Correspondence to Yi-Zeng Liang.

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Huang, LF., Li, BY., Liang, YZ. et al. Application of combined approach to analyze the constituents of essential oil from Dong quai. Anal Bioanal Chem 378, 510–517 (2004). https://doi.org/10.1007/s00216-003-2309-z

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  • DOI: https://doi.org/10.1007/s00216-003-2309-z

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