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Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors

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Abstract

This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERα, hPR and hAR). The tested chemicals are organochlorine insecticides (DDT and its metabolites, methoxychlor, aldrin, dieldrin, chlordecone, lindane, trichlorobenzene), estrogenic insecticides (endosulfan, toxaphene, nonachlor), herbicides (alachlor and atrazine), fungicides (benomyl and vinclozolin), industrial chemicals (nonylphenol, bisphenol A, diphenylphtalate), antioxidants (butylated hydroxyanisol) and some phytoestrogens. Except for phytoestrogens, most of the tested chemicals (DDT and its metabolites, aldrin, α- and β-endosulfan, toxaphen, trans-nonachlor) show higher affinities for hPR than for hERα, indicating that the interaction with the progesterone receptor could contribute to the endocrine-disrupting effects imputed to these chemicals. We propose to use binding assays using recombinant human steroid receptors as screening tools for the detection of endocrine disruptors in various samples.

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Authors and Affiliations

  1. Département des Sciences des Denrées Alimentaires, Université de Liège, Bâtiment B-43bis, Sart Tilman, 4000, Liège, Belgium

    Marie-Louise Scippo, Catherine Argiris & Guy Maghuin-Rogister

  2. Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de Liège, Bâtiment de Chimie B-6, Sart Tilman, 4000, Liège, Belgium

    Cécile Van De Weerdt, Marc Muller, Philippe Willemsen & Joseph Martial

Authors
  1. Marie-Louise Scippo
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  2. Catherine Argiris
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  3. Cécile Van De Weerdt
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  4. Marc Muller
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  5. Philippe Willemsen
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  6. Joseph Martial
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  7. Guy Maghuin-Rogister
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Correspondence to Marie-Louise Scippo.

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Scippo, ML., Argiris, C., Van De Weerdt, C. et al. Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors. Anal Bioanal Chem 378, 664–669 (2004). https://doi.org/10.1007/s00216-003-2251-0

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  • DOI: https://doi.org/10.1007/s00216-003-2251-0

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