Abstract
Several animal models have been developed to investigate osteoarthritis and potential disease-modifying therapeutics. However, early disease data from these models are limited by the resolution of current imaging modalities. In this in-vitro study, an optical coherence tomography (OCT) system with an axial resolution of 15 µm was used to track sequential changes in osteoarthritic rat knees. Osteoarthritis was induced via transection of the medial collateral ligament and an artificial full thickness meniscal tear. Imaging occurred at one, two, and three weeks after surgery. OCT successfully detected early signs of osteoarthritic change, including alteration of the cartilage surface and disruption of the bone–cartilage interface. This study demonstrates that OCT, along with the induction of mechanical injury, provides an excellent model for monitoring the sequential changes of osteoarthritis.
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References
Marijnissen AC, Van Roermund PM, TeKoppele JM, Bijlsma JW, Lafeber FP (2002) Osteoarthr Cartilage 10:145–155
Little CB, Ghosh P, Bellenger CR (1996) J Orthop Res 14:433–444
Lefkoe TP, Trafton PG, Ehrlich MG, et al (1993) J Orthop Trauma 7:458–467
Janusz MJ, Bendele AM, Brown KK, Taiwo YO, Hsieh L, Heitmeyer SA (2002) Osteoarthr Cartilage 10:785–791
Itay SA, Abramavici A, Zero Z (1987) Clin Orthop 220:284–303
Howell DS, Altman RD (1993) Rheum Dis Clin North Am 19:713–724
Brittberg M, Lindahl A, Nilsson A, et al (1994) New Eng J Med 331:879–895
Newman AP (1998) Am J Sports Med 26:309
Tyler JA, Watson PJ, Koh HL, Herrod NJ, Robson M, Hall LD (1995) Acta Orthop Scand 66(Suppl 2):130–138
Kim HK, Babyn PS, Harasiewicz, Gahunia HK, Pritzker KP, Foster FS (1995) J Orthop Res 13:963–970
Ayral X, Gueguen A, Ike RW, et al (1998) Osteoarthr Cartilage 6:160–166
Puliatifo CA, Hee MR, Schumann JS, Fujimoto JG (1995) Optical coherence tomography of ocular diseases. Slack, Thorofare, NJ
Brezinski ME, Tearney GJ, Bouma BE, et al (1996) Circulation 93:1206–1213
LightLab Imaging, Westford, MA, 2000–2003www.lightlabimaging.com
Tearney GJ, Brezinski ME, Bouma BE, et al (1997) Science 276:2037–2039
Huang D, Swanson EA, Lin CP, et al (1991) Science 254:1178–1181
Rumph PS, Kincaid SA, Baird DK, Kammermann JR, Visco DM, Goetze LF (1993) Am J Vet Res 54:365–369
Lane LB, Bullough PG (1980) J Bone Joint Surg 62B:372–375
Dmitrovsky E, Lane LB, Bullough PG (1978) Metab Bone Dis Related Res 1:115–136
Bullough PG, Jagannath A (1983) J Bone Joint Surg 65B:72–78
Green WT, Martin GN, Eanes ED, Sokoloff L (1970) Arch Pathol 90:151–158
Revell PA, Pirie C, Amir G, Rashad S, Walker F (1990) Rheumatol Int 10:143–147
Marshall LB, Roe M, Aspden RM (1999) J Anat 195:101–110
Venn M, Maroudas A (1977) Ann Rheum Dis 36:121–129
Acknowledgments
We would like to thank Eli Lilly and Company for their support of this program. In addition, funding was contributed by the National Institutes of Health, Contracts NIH-RO1-AR44812, NIH R01 AR46996, NIH R01- HL63953, NIH-1-R01-HL55686, and NIH R01 EB00419. Finally, thanks to Shawn J. Smith for his surgical efforts.
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Roberts, M.J., Adams, S.B., Patel, N.A. et al. A new approach for assessing early osteoarthritis in the rat. Anal Bioanal Chem 377, 1003–1006 (2003). https://doi.org/10.1007/s00216-003-2225-2
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DOI: https://doi.org/10.1007/s00216-003-2225-2