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Screen-printed multienzyme arrays for use in amperometric batch and flow systems

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Abstract

Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.

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Acknowledgements

Financial support from the European Commission (INTELLISENS, contact number: QLK3-2000-01481; MEBFOOD: IC15-CT98-0910), the Swedish National Research Council (Vetenskapsrådet), the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), and the Swedish Council for Forestry and Agricultural Research (SJFR) is gratefully acknowledged.

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Author notes

  1. S. Sapelnikova

    Present address: Bashkir State University, 32 Frunze Str., 450074, Ufa, Russia

Authors and Affiliations

  1. Department of Analytical Chemistry, Lund University, P.O. Box 124, 22 100 , Lund, Sweden

    S. Sapelnikova, E. Dock, R. Solná, T. Ruzgas & J. Emnéus

  2. Department of Biochemistry, Masaryk University, Kotlářská 2, 61137 , Brno, Czech Republic

    R. Solná & P. Skládal

Authors
  1. S. Sapelnikova
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  2. E. Dock
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  3. R. Solná
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  4. P. Skládal
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  5. T. Ruzgas
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  6. J. Emnéus
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Correspondence to S. Sapelnikova.

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Sapelnikova, S., Dock, E., Solná, R. et al. Screen-printed multienzyme arrays for use in amperometric batch and flow systems. Anal Bioanal Chem 376, 1098–1103 (2003). https://doi.org/10.1007/s00216-003-2000-4

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  • DOI: https://doi.org/10.1007/s00216-003-2000-4

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