Abstract
This study compares the behaviour of direct and mediated electrochemistry of horseradish peroxidase (HRP) immobilised on screen-printed carbon electrodes (SPCEs), screen-printed carbon electrodes modified with carboxyl-functionalised multi-wall carbon nanotubes (MWCNT-SPCEs) and screen-printed carbon electrodes modified with carboxyl-functionalised single-wall carbon nanotubes (SWCNT-SPCEs). The techniques of cyclic voltammetry and amperometry in the flow mode were used to characterise the properties of the HRP immobilised on screen-printed electrodes. From measurements of the mediated and mediatorless currents of hydrogen peroxide reduction at the HRP-modified electrodes, it was concluded that the fraction of enzyme molecules in direct electron transfer (DET) contact with the electrode varies substantially for the different electrodes. It was observed that the screen-printed carbon electrodes modified with carbon nanotubes (MWCNT-SPCEs and SWCNT-SPCEs) demonstrated a substantially higher percentage (≈100 %) of HRP molecules in DET contact than the screen-printed carbon electrodes (≈60 %). The HRP-modified electrodes were used for determination of hydrogen peroxide in mediatorless mode. The SWCNT-SPCE gave the lowest detection limit (0.40 ± 0.09 μM) followed by MWCNT-SPCE (0.48 ± 0.07 μM) and SPCE (0.98 ± 0.2 μM). These modified electrodes were additionally developed for amperometric determination of phenolic compounds. It was found that the SWCNT-SPCE gave a detection limit for catechol of 110.2 ± 3.6 nM, dopamine of 640.2 ± 9.2 nM, octopamine of 3341 ± 15 nM, pyrogallol of 50.10 ± 2.9 nM and 3,4-dihydroxy-l-phenylalanine of 980.7 ± 8.7 nM using 50 μM H2O2 in the flow carrier.
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References
Ruzgas T, Gorton L, Emnéus J, Marko Varga G (1995) J Electroanal Chem 391:41–49
Veitch NC (2004) Phytochemistry 65:249–259
Dzyadevych SV, Arkhypova VN, Soldatkin AP, Elskaya AV, Martelet C, Jaffrezic-Renault N (2008) ITBM-RBM 29:171–180
Habermüller K, Mosbach M, Schuhmann W (2000) J Anal Chem 366:560–568
Battistuzzi G, Stampler J, Bellei M et al (2011) Biochemistry 50:7987–7994
Lindgren A, Ruzgas T, Gorton L, Csoregi E, Ardila GB, Sakharov IY, Gazaryan IG (2000) Biosens Bioelectron 15:491–497
Lindgren A, Emneus J, Ruzgas T, Gorton L, Marko-Varga G (1997) Anal Chim Acta 347:51–62
Konash A, Magner E (2006) Biosens Bioelectron 22:116–123
Periasamy AP, Yang S, Chen SM (2011) Talanta 87:15–23
Yang Y, Yang G, Huang Y, Bai H, Lu X (2009) Colloids Surf A 340:50–55
Wang S, Xie F, Liu G (2009) Talanta 77:1343–1350
Xiang C, Zou Y, Sun LX, Xu F (2009) Sens Actuators B 136:158–162
Wang Y, Ma X, Wen Y, Xing Y, Zhang Z, Yang H (2010) Biosens Bioelectron 25:2442–2446
Astuti Y, Topoglidis E, Cass AG, Durrant JR (2009) Anal Chim Acta 648:2–6
Liu J, Li Y, Huang X, Zhu Z (2010) Nanoscale Res Lett 5:1177–1181
He P, Hu N (2004) Electroanalysis 16:1122–1131
Ansari AA, Solanki PR, Malhotra BD (2009) J Biotechnol 142:179–184
Mohammadi A, Moghaddam AB, Kazemzad M, Dinarvand R, Badraghi J (2009) Mater Sci Eng C 29:1752–1758
Yang X, Chen X, Zhang X, Yang W, Evans DG (2008) Sens Actuators B 134:182–188
Zong S, Cao Y, Zhou Y, Ju H (2006) Langmuir 22:8915–8919
Periasamy AP, Ting SW, Chen SM (2011) Int J Electrochem Sci 6:2688–2709
Yaropolov AI, Tarasevich MR, Vorfolomeev SD (1978) Bioelectrochem Bioenerg 5:18–24
Kong YT, Boopathi M, Shim YB (2003) Biosens Bioelectron 19:227–232
Jiangwen L, Lihong L, Fei X, Hhe G, Rui Y, Faqiong Z, Ling H, Baizhao Z (2008) J Electroanal Chem 613:51–57
Song Y, Wang L, Ren C, Zhu G, Li Z (2006) Sens Actuat B 114:1001–1006
Zhongqiang T, Ruo Y, Yaqin C, Shihong C, Yi X (2007) Biotechnol Lett 29:791–795
Adeyoju O, Iwuoha EI, Smyth MR (1994) Anal Proc 31:177–179
Comtat M, Durliat H (1994) Biosens Bioelectron 9:663–668
Lindgren A, Munteanu FD, Gazayan IG, Ruzgas T, Gorton L (1998) J Electroanal Chem 458:113–120
Huang R, Hu N (2001) Bioelectrochemistry 54:75–81
Tarasevich MR, Bogdanovskaya VA, Fridman VA, Kuznetsova LN (2001) Russ J Electrochem 37:730–734
Tatsuma T, Mori H, Fujishima A (2000) Anal Chem 72:2919–2924
Hart JP, Wring SA (1994) Electroanalysis 6:617–624
Hart JP, Wring SA (1997) Trends Anal Chem 16:89–103
Honeychurch KC, Hart JP (2003) Trends Anal Chem 22:456–469
Merkoci A, Pumera M, Llopis X, Perez B, Del Valle M, Alegret S (2005) Trends Anal Chem 24:826–838
Wang J, Liu G, Jan MR (2004) J Am Chem Soc 126:3010–3011
Robertson J (2004) Mater Today 7:46–52
Luo H, Shi Z, Li N, Gu Z, Zhuang Q (2001) Anal Chem 73:915–920
Musameh M, Wang J, Merkoc i A, Lin Y (2002) Electrochem Commun 4:743–746
García-González R, Fernández-Abedul MT, Pernía A, Costa-García A (2008) Electrochim Acta 53:3242–3249
Lindgren A, Tanaka M, Ruzgas T, Gorton L, Gazaryan I, Ishimori K, Morishima I (1999) Electrochem Commun 1:171–175
Elkaoutit M, Naranio-Rodrigueza I, Domínguez M, Hernandez-Artig MP, Bellido-Milla D, De Cisnerosa JLHH (2008) Electrochim Acta 53:7131–7137
Liu CY, Hu JM (2008) Chin Chem Lett 19:955–958
Lin J, Zhang L, Zhang S (2007) Anal Biochem 370:180–185
Yao H, Li N, Xu S, Xu JZ, Zhu JJ, Chen HY (2005) Biosens Bioelectron 21:372–377
Chen S, Yuan R, Chai Y, Yin B, Li W, Min L (2009) Electrochim Acta 54:3039–3046
Teng YJ, Zuo SH, Lan MB (2009) Biosens Bioelectron 24:1353–1357
Rosatto SS, Sotomayor PT, Kubota LT, Gushikem Y (2002) Electrochim Acta 47:4451–4458
Santos AS, Pereira AC, Sotomayor MDPT, Tarley CRT, Durán N, Kubota LT (2007) Electroanalysis 19:549–554
Munteanu FD, Lindgren A, Emnéus J, Gorton L, Ruzgas T, Csöregi E, Ciucu A, Huystee RBV, Gazaryan IG, Lagrimini LM (1998) Anal Chem 70:2596–2600
Li J, Xiao LT, Liu XM, Zeng GM, Huang GH, Shen GL, Yu RQ (2003) Anal Bioanal Chem 376:902–907
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Chekin, F., Gorton, L. & Tapsobea, I. Direct and mediated electrochemistry of peroxidase and its electrocatalysis on a variety of screen-printed carbon electrodes: amperometric hydrogen peroxide and phenols biosensor. Anal Bioanal Chem 407, 439–446 (2015). https://doi.org/10.1007/s00216-014-8282-x
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DOI: https://doi.org/10.1007/s00216-014-8282-x