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A validated spectrofluorometric method for the determination of celecoxib in capsules

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Abstract.

A rapid, selective, sensitive and simple fluorescence method was developed for the direct determination of celecoxib in capsules. The capsules were emptied, pulverized and dissolved in either ethanol or acetonitrile, sonicated and filtered. Direct fluorescence emission was measured at 355±5 nm (exciting at 272 nm). The method was fully validated and the recoveries were excellent, even in presence of excipients.

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Acknowledgements.

Financial support of the Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Agencia Nacional de Promoción Científica y Tecnológica are gratefully acknowledged. The authors also thank Dr Alejandro Olivieri who revised our manuscript and suggested suitable modifications and Dr Héctor Girolami who helped us to make the HPLC analysis. We also wish to thank Searle Syntial and Monsanto Argentina SAIC for the supply of the pharmaceutical preparations and the reference standard of celecoxib.

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Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario , Argentina

    Patricia Damiani, Mariela Bearzotti & Miguel A. Cabezón

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  1. Patricia Damiani
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  2. Mariela Bearzotti
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  3. Miguel A. Cabezón
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Correspondence to Patricia Damiani.

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Damiani, P., Bearzotti, M. & Cabezón, M.A. A validated spectrofluorometric method for the determination of celecoxib in capsules. Anal Bioanal Chem 376, 1141–1146 (2003). https://doi.org/10.1007/s00216-003-1975-1

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  • DOI: https://doi.org/10.1007/s00216-003-1975-1

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