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Isolation and determination of p-hydroxybenzoylcholine in traditional Chinese medicine Semen sinapis Albae

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Abstract.

A special component is isolated from Semen sinapis Albae (white mustard seed), a traditional Chinese medicine. According to the physical and chemical investigation and spectroscopic identification, this component can be known as p-hydroxybenzoylcholine bisulfate, a choline base. This component in the drug is also determined by RP-HPLC. A reversed-phase C18 column is used to separate the p-hydroxybenzoylcholine with an eluent of methanol–0.05 mol/L monopotassium phosphate solution (30:70) (adjusted by phosphoric acid to pH 3.6) at the flow rate of 0.5 mL/min. Detection is carried out with a UV detector operated at 285 nm, and the column temperature is 25 °C. It reveals that there is 0.021% (w/w) of p-hydroxybenzoylcholine bisulfate in Semen sinapis Albae and 0.037% (w/w) in stir-baked Semen sinapis Albae.

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Authors and Affiliations

  1. Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093, Nanjing, P. R. China

    Lifang Liu, Tao Liu & Genxi Li

  2. Department of Pharmacognosy, China Pharmaceutical University, 210009, Nanjing, P. R. China

    Lifang Liu & Qiang Wang

  3. Department of Biochemistry, The Chinese University of Hongkong, Shatin, Hongkong, P. R. China

    Tzibun Ng

Authors
  1. Lifang Liu
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  2. Tao Liu
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  3. Genxi Li
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  4. Qiang Wang
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  5. Tzibun Ng
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Correspondence to Genxi Li.

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Liu, L., Liu, T., Li, G. et al. Isolation and determination of p-hydroxybenzoylcholine in traditional Chinese medicine Semen sinapis Albae. Anal Bioanal Chem 376, 854–858 (2003). https://doi.org/10.1007/s00216-003-1964-4

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  • DOI: https://doi.org/10.1007/s00216-003-1964-4

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