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Quantification of bile acids directly from urine by MALDI–TOF–MS

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Abstract

The ability to quantify mixtures of bile acids using matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) mass spectrometry directly from urine has been demonstrated. Six cholic acid derivatives were selected for analysis: taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), taurolithocholic acid (TLCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), and glycolithocholic acid (GCDCA). Urine samples were pre-concentrated and purified using solid-phase extraction (SPE) columns. The method was optimized to eliminate suppression effects, and proved to be reproducible from day to day. Calibration curves averaged from three days were obtained for the bile acids directly from urine, and then tested for their ability to accurately determine concentrations from one measurement. In summary, a simple, rapid method has been developed for the quantification of bile salts from urine with SPE clean-up by MALDI-MS.

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Acknowledgements

The authors thank Rachal Vermillion-Salsbury and Dr Deborah Hunka for valuable scientific discussion, and the National Science Foundation for financial support, under grant CHE-9985864.

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  1. Department of Chemistry, Vanderbilt University, Nashville, TN, 37214, USA

    DebraLynn Mims & David Hercules

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  1. DebraLynn Mims
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  2. David Hercules
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Correspondence to David Hercules.

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Mims, D., Hercules, D. Quantification of bile acids directly from urine by MALDI–TOF–MS. Anal Bioanal Chem 375, 609–616 (2003). https://doi.org/10.1007/s00216-003-1771-y

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