Abstract
Bile acid (BA) has attracted significant attention because it is considered a metabolic regulator in the body. BA metabolism is modulated by dietary intervention and disease conditions. Here, we present a comprehensive BA measurement system used to enable an understanding of the BA distribution in the body. We also focused on BA extraction methods, such as organ, fluid, or semisolid samples, depending on sample characteristics. To precisely measure the BA composition, we introduced an ultra-high-performance liquid chromatography/orbitrap-mass spectrometry (UHPLC-Orbitrap MS) technique for BA analysis. This method enabled us to measure BA concentrations in various biological samples derived from experimental animals and humans similarly, and would be a useful tool for investigating the roles of BA in physiological and pathological events.
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Hori, S. et al. (2022). Extraction of Bile Acids from Biological Samples and Quantification Using Ultra-High-Performance Liquid Chromatography-Orbitrap Mass Spectrometry. In: Betim Cazarin, C.B. (eds) Basic Protocols in Foods and Nutrition. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2345-9_8
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DOI: https://doi.org/10.1007/978-1-0716-2345-9_8
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