Abstract
Laryngeal squamous cell carcinoma (LSCC) is the most common type of laryngeal cancer with poor prognosis. In the present study, we aimed to investigate the biological role of long non-coding RNA OIP5-AS1 in LSCC. The results demonstrated that the expression levels of OIP5-AS1 were significantly increased in LSCC tissues and cell lines. High expression of OIP5-AS1 was closely correlated with lymph node metastasis and advanced clinical stage of LSCC patients. Moreover, in vitro assays showed that OIP5-AS1 overexpression promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of LSCC cells, whereas OIP5-AS1 knockdown exerted suppressive effects on LSCC cells. Furthermore, OIP5-AS1 was confirmed to serve as a competing endogenous RNA of miR-204-5p in LSCC cells, and restoration of miR-204-5p counteracted the OIP5-AS1-mediated oncogenic effects. In conclusion, our study provides promising evidence that lncRNA OIP5-AS1 functions as a tumor promoter in LSCC and may be used as a potential target for LSCC therapy.
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Hui Wang and Ben Ye conceived the study and designed the experiments. Hui Wang, Jiantong Qian, and Xiaojing Xia performed the experiments and analyzed the data. Hui Wang and Ben Ye wrote the article. All authors read and approved the final manuscript.
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This study was approved by the Ethics Committee of Heze Mudan People’s Hospital and conducted in accordance with the Declaration of Helsinki. Written informed consents were obtained from all patients.
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Wang, H., Qian, J., Xia, X. et al. Long non-coding RNA OIP5-AS1 serves as an oncogene in laryngeal squamous cell carcinoma by regulating miR-204-5p/ZEB1 axis. Naunyn-Schmiedeberg's Arch Pharmacol 393, 2177–2184 (2020). https://doi.org/10.1007/s00210-020-01811-7
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DOI: https://doi.org/10.1007/s00210-020-01811-7