Abstract
A variety of Cu reconstituted metallothioneins (MTs) containing different amounts of copper ions together with Cd7-MT free of copper were prepared and used in spin trapping experiments designed to show cadmium is not a Fenton active metal. A significant increase of the DMPO/·OH adduct was observed, with increased concentrations of the copper containing MTs, H2O2 enhanced DMPO/·OH adduct formation, catalase and the Cu (I) specific chelating agent bathocuproine, reduced DMPO/·OH adduct formation. These results suggest that Cu (I) and H2O2 both have important roles in the production of active species in these systems and cause DMPO/·OH formation. However, Cd7-MT showed no ability to cause generation of DMPO adducts with H2O2 seeming to indicate cadmium is not a Fenton metal. To test this hypothesis further trapping studies were run with added sulphite and lipid peroxide using both commercial MT and Cd7-MT since cadmium causes peroxidation in vivo. Commercial MT generates radicals with added sulphite and peroxide, Cd7-MT does not, demonstrating that cadmium is not a Fenton metal. These results help to explain the oxidative damage to DNA observed in the presence of MT and cadmium in vitro.
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Received: 3 February 1998 / Accepted: 24 July 1998
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O'Brien, P., Salacinski, H. Evidence that the reactions of cadmium in the presence of metallothionein can produce hydroxyl radicals. Arch Toxicol 72, 690–700 (1998). https://doi.org/10.1007/s002040050562
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DOI: https://doi.org/10.1007/s002040050562