Abstract
The soluble epoxide hydrolase mRNA level in liver was increased eight-fold upon administration of the hypolipidemic drug and peroxisome proliferator clofibrate for 7 days to mice. The soluble epoxide hydrolase mRNA was back at control levels within 1–2 days after clofibrate withdrawal. The highest expression was in liver, intestine and kidney. Lower levels were found in heart and muscle and very low levels were found in testes, lung, brain and spleen. The mRNA levels were increased in liver, kidney and heart by clofibrate.
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References
Arand M, Knehr M, Thomas H, Zeller HD, Oesch F (1991) An impaired peroxisomal targeting sequence leading to an unusual bicompartmental distribution of cytosolic epoxide hydrolase. FEBS Lett 294: 19–22
Ehrenberg L, Hussein S (1981) Genetic toxicity of some important epoxides. Mutat Res 86: 1–113
Grant DF, Storms DH, Hammock BD (1993) Molecular cloning and expression of murine liver soluble epoxide hydrolase. J Biol Chem 268: 17628–17633
Joste V, Meijer J (1989) In vitro translation of cytosolic and peroxisomal epoxide hydrolase and catalase on liver polyribosomes from untreated and clofibrate-treated C57Bl/6 mice. FEBS Lett 249: 83–88
Karlsson R (1988) Expression of chicken beta-actin in Saccharomyces cerevisiae. Gene 68: 249–257
Knehr M, Thomas H, Arand M, Gebel T, Zeller HD, Oesch F (1993) Isolation and characterization of a cDNA encoding rat liver cytosolic epoxide hydrolase and its functional expression in Escherichia coli. J Biol Chem 268: 17623–17627
Lazarow PB, Fujiki Y (1985) Biogenesis of peroxisomes. Annu Rev Cell Biol 1: 489–530
Loury DN, Moody DE, Kim BW, Hammock BD (1985) Effect of dietary clofibrate on epoxide hydrolase activity in tissues of mice. Biochem Pharmacol 34: 1827–1833
Meijer J, DePierre JW (1985) Properties of cytosolic epoxide hydrolase purified from the liver of untreated and clofibrate treated mice. Purification procedure and physicochemical characterization of the pure enzymes. Eur J Biochem 148: 421–430
Meijer J, DePierre JW (1988) Cytosolic epoxide hydrolase. Chem-Biol Interact 64: 207–249
Meijer J, Starkerud C, Granell I, Afzelius BA (1991) Time dependent effects of the hypolipidemic agent clofibrate on peroxisomes and mitochondria in mouse hepatocytes. J Submicroscop Cytol Pathol 23: 185–194
Meijer J, Starkerud C, Afzelius BA (1993) Effects of clofibrate withdrawal on peroxisomes in mouse hepatocytes. Eur J Cell Biol 60: 291–299
Riviere LR, Fleming M, Elicone G, Tempst P (1992) Study and applications of the effects of detergents and chaotropes on enzymatic proteolysis. In: Villafranca JJ (ed) Techniques in protein chemistry II. Academic Press, New York
Tso JY, Sun X-H, Kao T-H, Reece KS, Wu R (1985) Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucl Acid Res 13: 2485–2502
Waechter F, Bentley P, Bieri F, Muakkassah-Kelly S, Stäubli W, Villermain M (1988) Organ distribution of epoxide hydrolases in cytosolic and microsomal fractions of normal and nafenopin-treated male DBA/2 mice. Biochem Pharmacol 37: 3897–3903
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Johansson, C., Stark, A., Sandberg, M. et al. Tissue specific basal expression of soluble murine epoxide hydrolase and effects of clofibrate on the mRNA levels in extrahepatic tissues and liver. Arch Toxicol 70, 61–63 (1995). https://doi.org/10.1007/s002040050250
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DOI: https://doi.org/10.1007/s002040050250