Abstract
Syrian Golden hamster dams were administered 203Hg-labelled methyl mercury (MeHg; 1.6 μmol/kg) 1 day after parturition and milk was collected twice during the 1st week. The excretion of 203Hg in milk and the uptake, retention and tissue distribution of 203Hg in the pups was studied using gamma counting. The fraction of inorganic Hg in milk and in the kidneys of the pups was determined following separation of inorganic Hg and MeHg by ion exchange chromatography. The concentration of 203Hg in milk on the 1st day after MeHg administration was 0.12 nmol/g. 203Hg was mainly (80–90%) excreted as MeHg during the first 6 days of lactation. The whole body and tissue concentration of 203Hg in the pups increased for 10–15 days and decreased thereafter. The content of 203Hg in the pelt and the fraction of inorganic Hg in the kidney increased throughout the study period (4 weeks). The excretion of MeHg in milk corresponded to at least 5% of the dose administered to the dam. Our study demonstrates that breast milk may be a significant source of MeHg exposure during the critical neonatal period.
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Amin-Zaki L, Elhassani S, Majeed MA, Clarkson TW, Doherty RA, Greenwood MR (1974) Studies of infants postnatally exposed to methylmercury. J Pediatr 85: 81–84
Amin-Zaki L, Elhassani S, Majeed MA, Clarksson TW, Doherty RA, Greenwood MR, Giovanoli-Jakubczak T (1976) Perinatal methylmercury poisoning in Iraq. Am J Dis Child 130: 1070–1076
Amin-Zaki L, Majeed MA, Greenwood MR, Elhassani SB, Clarksson TW, Doherty RA (1981) Methylmercury poisoning in the Iraqi suckling infant: a longitudinal study over five years. J Appl Toxicol 1: 210–214
Aschner M, Aschner JL (1990) Mercry neurotoxicity: mechanisms of blood-brain barrier transport. Neurosci Biobehav Rev 14 [2]: 169–176
Bakir F, Damluji SF, Amin-Zaki L, Murtadha M, Khalidi A, Al-Rawi NY, Tikriti S, Dhahir HI, Clarkson TW, Smith JC, Doherty RA (1973) Methylmercury poisoning in Iraq. Science 181: 230–241
Choi BH (1989) The effects of methylmercury on the developing brain. Prog Neurobiol 32: 447–470
Dobbing J, Sands J (1973) Quantitative growth and development of human brain. Arch Dis Child 48: 757–767
Dock L, Rissanen R-L, Vahter M (1994) Demethylation and placental transfer of methyl mercury in the pregnant hamster. Toxicology 94: 131–142
Dock L, Rissanen R-L, Vahter M (1995) Metabolism of mercury in hamster pups administered a single dose of 203Hg-labeled methyl mercury. Pharmacol Toxicol 76: 80–84
Doherty RA, Gates AH, Landry TD (1977) Methylmercury excretion: developmental changes in mouse and man. Pediat Res 11: 416
Falkmer S, Waller T (1984) Försöksdjursteknik. Almqvist & Wiksell, Stockholm, p 70 (in Swedish)
Greenwood MR, Clarkson TW, Doherty RA, Gates AH, Amin-Zaki L, Elhassani S, Majeed MA (1978) Blood clearance half-times in lactating and nonlactating members of a population exposed to methylmercury. Environ Res 16: 48–54
Habermann BH (1974) Mechanical collection from mice for Bittner virus isolation. Lab Anim Sci 24: 935–937
Kjellström T, Kennedy P, Wallis S, Stewart A, Friberg L, Lind B, Wutherspoon P, Mantell C (1989) Physical and mental development of children with prenatal exposure to mercury from fish. Stage 2: interviews and psychological tests at age 6. National Swedish Environmental Protection Board Report 3642
Kostial K, Kello D, Jugo S, Rabar I, Maljkovic T (1978) Influence of age on metal metabolism and toxicity. Environ Health Perspect 25: 81–86
Kostial K, Simonovic I, Rabar I, Blanusa M, Landeka M (1983) Age and intestinal retention of mercury and cadmium in rats. Environ Res 31: 111–115
Lok E (1983) The effect of weaning on blood, hair, fecal and urinary mercury after chronic ingestion of methylmercuric chloride by infant monkeys. Toxicol Lett 15: 147–152
May K, Stoeppler M, Reisinger K (1987) Studies in the ratio total mercury/methylmercury in the aquatic food chain. Toxicol Environ Chem 13: 153–159
Miettinen JK (1973) Absorption and elimination of dietary mercury (Hg2+) and methylmercury in man. In: Miller MW, Clarkson TW (eds) Mercury, mercurials and mercaptans. Charles C Thomas, Springfield Ill. pp 233–243
Naganuma A, Koyama Y, Imura N (1980) Behavior of methylmercury in mammalian erythrocytes. Toxicol Appl Pharmacol 54: 405–410
Naganuma A, Urano T, Imura N (1985) A simple and rapid method for preparation of 203Hg-labeled methylmercury from 203HgCl2 and methylcobalamin. J Pharmacobiodyn 8: 69–72
Nagasawa H (1979) A device for milk collection from mice. Lab Anim Sci 29: 633–635
Omata S, Kasama H, Hasegawa H, Hasegawa K, Ozaki K, Sugano H (1986) Species difference between rat and hamster in tissue accumulation of mercury after administration of methylmercury. Arch Toxicol 59: 249–254
Oskarsson A (1987) Effect of disulfiram on milk transfer and tissue distribution of lead in the neonatal rat. Toxicol Lett 36: 73–79
Petersson K, Dock L, Vahter M (1989) Metabolism of methylmercury in rabbits and hamsters. Biol Trace Elem Res 21: 219–226
Petersson K, Dock L, Söderling K, Vahter M (1991) Distribution of mercury in rabbits subchronically exposed to low levels of radiolabeled methylmercury. Pharmacol Toxicol 68: 464–468
Renshaw HW, Van Hoosier GL, Amend NK (1975) A survey of naturally occuring diseases of the Syrian hamster. Lab Anim 9: [3]: 179–191
Rowland IR, Robinson RD, Doherty RA, Landry TD (1983) Are developmental changes in methylmercury metabolism and excretion mediated by the intestinal microflora? In: Clarkson TW, Nordberg GF, Sager PR (eds) Reproductive and developmental toxicity of metals. Plenum Press, New York, pp 745–758
Shi C, Lane AT, Clarkson TW (1990) Uptake of mercury by the hair of methylmercury-treated newborn mice. Environ Res 51: 170–181
Skerfving S (1988) Mercury in women exposed to methylmercury through fish consumption, and in their newborn babies and breast milk. Bull Environ Contam Toxicol 41: 475–482
Sundberg J (1992) Environmental contaminants in breast milk. Vår Föda 44: 250–254
Sundberg J, Oskarsson A, Albanus L (1991) Methylmercury exposure during lactation: milk concentration and tissue uptake of mercury in the neonatal rat. Bull Environ Contam Toxicol 46: 255–262
Sundberg J, Oskarsson A (1992) Placental and lactational transfer of mercury from rats exposed to methylmercury in their diet: speciation of mercury in the offspring. J Trace Elem Exp Med 5: 47–56
Swedish Expert Group (1971) Methylmercury in fish. A toxicologicalepidemiological evaluation of risks. Nord Hyg Tidskr 4[Suppl]:19–364
Thomas DJ, Fisher HL, Hall IL, Mushak P (1982) Effects of age and sex on retention of mercury by methyl mercury-treated rats. Toxicol Appl Pharmacol 62: 445–454
Thomas DJ, Fisher HL, Sumler MR, Hall IL, Mushak P (1988) Distribution and retention of organic and inorganic mercury in methyl mercury-treated neonatal rats. Environ Res 47: 59–71
Weihe WH (1968) Der Milchleistungstest bei Versuchstieren. Z Versuchsitierk 10: 147–157
WHO (1990) Environmental Health Criteria 101: methylmercury. World Health Organization, Geneva
Yoshida M, Satoh H, Kishimoto T, Yamamura Y (1992) Exposure to mercury via breast milk in suckling offspring of maternal guinea pigs exposed to mercury vapor after parturition. J Toxicol Environ Health 35: 135–139
Yoshida M, Watanabe C, Satoh H, Kishimoto T, Yamamura Y (1994) Milk transfer and tissue uptake in suckling offspring after exposure of lactating maternal guinea pigs to inorganic or methylmercury. Arch Toxicol 68: 174–178
Åberg B, Ekman L, Falk R, Greitz U, Persson G, Snihs J-O (1969) Metabolism of methylmercury (203Hg) compounds in man. Arch Environ Health 19: 478–484
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Nordenhäll, K., Dock, L. & Vahter, M. Lactational exposure to methylmercury in the hamster. Arch Toxicol 69, 235–241 (1995). https://doi.org/10.1007/s002040050164
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DOI: https://doi.org/10.1007/s002040050164