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Effect of cadmium (CdCl2) on cell proliferation and production of EDRF (endothelium-derived relaxing factor) by cultured human umbilical arterial endothelial cells

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Abstract

The effect of cadmium chloride (CdCl2) on cell proliferation and EDRF (endothelium-derived relaxing factor) production by cultured human umbilical arterial endothelial cells (HUAECs) was investigated. The viability of HUAECs decreased dose-dependently after the addition of Cd (cadmium chloride). Morphologic examination by phase contrast microscopy revealed severe damaging effects of Cd at higher concentrations. The cytotoxic effect of Cd on DNA synthesis was also concentration-dependent. The effect of Cd on EDRF production by indomethacin-treated HUAECs was assessed by its anti-platelet aggeragory effect. Platelet aggregation studies were carried out in cuvettes lined with HUAECs using an aggregometer. The anti-platelet aggregatory effect was decreased dose-dependently by pretreatment with Cd. These findings suggest that HUAECs are susceptible to concentration-dependent Cd cytotoxicity, and that Cd can inhibit the production of EDRF by HUAECs.

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Authors and Affiliations

  1. Department of Environmental Medicine, Shimane Medical University, 693, Izumo, Japan

    Takuji Kishimoto, Tetsuhisa Oguri & Manabu Tada

  2. Department of Dermatology, Shimane Medical University, 693, Izumo, Japan

    Mika Ohno

  3. Department of Legal Medicine, Shimane Medical University, 693, Izumo, Japan

    Kazuo Matsubara

  4. Department of Obstetrics and Gynecology, Shimane Medical University, 693, Izumo, Japan

    Kazuhiko Yamamoto

Authors
  1. Takuji Kishimoto
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  2. Tetsuhisa Oguri
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  3. Mika Ohno
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  4. Kazuo Matsubara
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  5. Kazuhiko Yamamoto
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  6. Manabu Tada
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Kishimoto, T., Oguri, T., Ohno, M. et al. Effect of cadmium (CdCl2) on cell proliferation and production of EDRF (endothelium-derived relaxing factor) by cultured human umbilical arterial endothelial cells. Arch Toxicol 68, 555–559 (1994). https://doi.org/10.1007/s002040050113

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  • DOI: https://doi.org/10.1007/s002040050113

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