Abstract
Mercaptobenzothiazole (MBT) is used while manufacturing natural rubber products. Our study deals with assessing its allergenic potential following dermal and oral routes of exposure, using a biphasic local lymph node assay (LLNA). Female Balb/c mice were treated with MBT (dermally 3, 10, 30% concentrations in DMSO; orally 1, 10, 100 mg/kg doses in corn oil) on the back (dermal study) or through oral administration (oral study) on days 1–3 followed by auricular application of 3, 10 and 30% concentrations, respectively, on days 15–17. End points determined on day 19 included ear thickness, ear punch weight, lymph node weight, lymph node cell count, and lymphocyte subpopulations (CD4+, CD8+, CD45+). After dermal application of 3% or 10% solution, a significant increase in cell count and lymph node weight along with significant decrease in CD8+ cells was observed. After initial oral administration of 1 mg/kg, we noticed a significant amplification in cell count. Following oral administration of 10 mg/kg, we observed a similar increase in cell count and lymph node weight. The results of our study show that the modified biphasic LLNA protocol can be used to study the sensitising potential of a compound also following the oral route of exposure.
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The financial support provided by Federal Institute for Risk Assessment (BfR, Berlin, Germany) is gratefully acknowledged.
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Ahuja, V., Wanner, R., Platzek, T. et al. Appraisal of the sensitising potential of orally and dermally administered Mercaptobenzothiazol by a biphasic protocol of the local lymph node assay. Arch Toxicol 83, 933–939 (2009). https://doi.org/10.1007/s00204-009-0426-y
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DOI: https://doi.org/10.1007/s00204-009-0426-y