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Investigation of the sensitising and cross-sensitising potential of textile dyes and β-lactam antibiotics using a biphasic mice local lymph node assay

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Abstract

We used a modified protocol of the murine local lymph node assay (LLNA) to study the cross-sensitising potential of (a) textile dye disperse yellow 3 and its metabolite 2-amino-p-cresol, (b) two antibiotics, penicillin G and cefotiam. The test substances were applied in a biphasic manner, i.e. first on the shaved skin of the back followed by application on the dorsal side of the ears after 2 weeks. The end-points analysed included thickness and weight of an ear-biopsy, weight and cell number of the draining lymph node, and lymphocyte cell surface markers analysed by flow-cytometry. Disperse yellow 3 and its metabolite significantly altered the various end-points at both the tested concentrations (0.5 and 1%), thus demonstrating the sensitising potential of the two substances. The cross-sensitisation study showed significant modulation in the tested variables in the treated group as compared to the control, signifying cross-sensitisation potential of the two substances. Penicillin G and cefotiam showed significant changes in various end-points, pointing towards their sensitising potential. However, even at 50% concentration of the β-lactams no significant change in any end-point indicating absence of cross-reactivity of the antibiotics was noticed. We conclude that a biphasic, modified protocol of the LLNA is a suitable approach to test for a cross-reactivity potential of two related compounds.

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Authors and Affiliations

  1. Department of Toxicology, Institute of Clinical Pharmacology and Toxicology, Charité Medical University, Campus Benjamin Franklin, Garystraße 5, 14195, Berlin, Germany

    Varun Ahuja, Clemens Schreiber & Ralf Stahlmann

  2. Federal Institute for Risk Assessment, Berlin, Germany

    Thomas Platzek

Authors
  1. Varun Ahuja
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  2. Clemens Schreiber
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  3. Thomas Platzek
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  4. Ralf Stahlmann
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Correspondence to Ralf Stahlmann.

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Ahuja, V., Schreiber, C., Platzek, T. et al. Investigation of the sensitising and cross-sensitising potential of textile dyes and β-lactam antibiotics using a biphasic mice local lymph node assay. Arch Toxicol 83, 691–699 (2009). https://doi.org/10.1007/s00204-009-0407-1

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  • DOI: https://doi.org/10.1007/s00204-009-0407-1

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