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Haplotypes of vitamin D receptor modulate the circulating levels of lead in exposed subjects

  • Inorganic Compounds
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Abstract

Genetic factors influence whole blood lead (Pb-B) concentrations in lead exposed subjects. This study aimed at examining the combined effects (haplotype analysis) of three polymorphisms (BsmI, ApaI and FokI) in vitamin D receptor (VDR) gene on Pb-B and on the concentrations of lead in plasma (Pb-P), which is more relevant to lead toxicity, in 150 environmentally exposed subjects. Genotypes were determined by RFLP, and Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. Subjects with the bb (BsmI polymorphism) or ff (FokI polymorphism) genotypes have lower B-Pb than subjects in the other genotype groups. Subjects with the aa (ApaI polymorphism) or ff genotypes have lower P-Pb than subjects in the other genotype groups. Lower Pb-P, Pb-B, and %Pb-P/Pb-B levels were found in subjects with the haplotype combining the a, b, and f alleles for the ApaI, BsmI, and FokI polymorphisms, respectively, compared with the other haplotype groups, thus suggesting that VDR haplotypes modulate the circulating levels of lead in exposed subjects.

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Acknowledgments

This study was funded by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP-Brazil) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil).

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, 13081-970, Campinas, SP, Brazil

    Vania B. Rezende

  2. Department of Clinical, Toxicological and Food Science Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Av. do Cafe s/n, 14040-903, Ribeirao Preto, SP, Brazil

    Fernando Barbosa Jr

  3. Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Av. Bandeirantes, 3900, Monte Alegre, CEP 14049-900, Ribeirao Preto, SP, Brazil

    Marcelo F. Montenegro, Valeria C. Sandrim & Jose E. Tanus-Santos

  4. Department of Morphology, Estomatology and Physiology, Dental School of Ribeirao Preto, University of Sao Paulo, Av. do Cafe s/n, 14040-904, Ribeirao Preto, SP, Brazil

    Raquel F. Gerlach

Authors
  1. Vania B. Rezende
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  2. Fernando Barbosa Jr
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  3. Marcelo F. Montenegro
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  4. Valeria C. Sandrim
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  5. Raquel F. Gerlach
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  6. Jose E. Tanus-Santos
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Correspondence to Jose E. Tanus-Santos.

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Rezende, V.B., Barbosa, F., Montenegro, M.F. et al. Haplotypes of vitamin D receptor modulate the circulating levels of lead in exposed subjects. Arch Toxicol 82, 29–36 (2008). https://doi.org/10.1007/s00204-007-0231-4

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  • DOI: https://doi.org/10.1007/s00204-007-0231-4

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