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Effects of naphthoquinone on airway responsiveness in the presence or absence of antigen in mice

  • Organ Toxicity and Mechanisms
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Abstract

We have recently demonstrated that naphthoquinone (NQ), one of extractable chemical compounds of diesel exhaust particles (DEP), enhances antigen-related airway inflammation with goblet cell hyperplasia in mice (Inoue et al. in Eur Respir J 209(2):259–267, 2007). Further, NQ has enhanced lung expressions of interleukin (IL)-4 and IL-5. However, the effects of NQ on other cardinal features of asthma have not been completely investigated. The aim of the present study was to evaluate the effects of NQ on airway responsiveness on the model. Vehicle, NQ, ovalbumin (OVA), or NQ + OVA was administered intratarcheally to ICR mice for 6 weeks. Twenty-four hours after the last instillation, lung histology, lung functions such as total respiratory system resistance (R) and Newtonian resistance (R n), and protein level of IL-13 and mRNA level for MUC5AC in the lung were examined. Repetitive exposure to NQ aggravated antigen-related lung inflammation. NQ alone enhanced R and R n as compared to vehicle without statistical significance. OVA alone or NQ plus OVA showed increases in R and R n, which was prominent in NQ plus OVA (P < 0.05 vs. vehicle). Combined exposure to NQ and OVA elevated the levels of IL-13 and MUC5AC in the lung as compared with exposure to NQ or OVA alone. These results indicate that NQ can enhance airway hyperresponsiveness in the presence or absence of an antigen. Also, amplified lung expressions of IL-13 and MUC5AC might partly contribute to the deterioration of asthma features by NQ.

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Authors and Affiliations

  1. Environmental Health Sciences Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

    Ken-ichiro Inoue, Hirohisa Takano, Rie Yanagisawa & Miho Sakurai

  2. Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan

    Shigeo Tomura & Kyoko Hiyoshi

  3. Department of Health Science, Oita University of Nursing and Health Science, Oita, Japan

    Takamichi Ichinose

  4. Department of Environmental Medicine, Institute of Community Medicine, University of Tsukuba, Tsukuba, Japan

    Daigo Sumi & Yoshito Kumagai

  5. Southern California Particle Center and Supersite, University of California, Los Angeles, USA

    Arthur K. Cho & Yoshito Kumagai

Authors
  1. Ken-ichiro Inoue
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  2. Hirohisa Takano
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  3. Takamichi Ichinose
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  4. Shigeo Tomura
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  5. Rie Yanagisawa
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  6. Miho Sakurai
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  7. Daigo Sumi
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  8. Arthur K. Cho
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  9. Kyoko Hiyoshi
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  10. Yoshito Kumagai
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Correspondence to Hirohisa Takano.

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Inoue, Ki., Takano, H., Ichinose, T. et al. Effects of naphthoquinone on airway responsiveness in the presence or absence of antigen in mice. Arch Toxicol 81, 575–581 (2007). https://doi.org/10.1007/s00204-007-0186-5

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  • DOI: https://doi.org/10.1007/s00204-007-0186-5

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