Effect of metal complexation on the bioavailability of nitrilotriacetic acid to Chelatobacter heintzii ATCC 29600

Abstract.

Many polluted sites contain a mixture of organics and heavy metals. Nitrilotriacetic acid has been chosen as a model organic compound to study the effect of metal binding on organic bioavailability and degradation of organics. The effect of varying the ratio of metal to nitrilotriacetic acid on its utilisation has been examined using the gram-negative bacterium Chelatobacter heintzii ATCC 29600. The following parameters of substrate utilisation were examined: growth, degradation, respiration, mineralisation and nitrilotriacetic acid uptake. Complexation of nitrilotriacetic acid by Cu(II), Ni(II), Co(II) and Zn(II) prevented utilisation of nitrilotriacetic acid by C. heintzii; complexation to Fe(III) or Mn(II) did not. The pattern of inhibition was consistent with a 1:1 stoichiometry of metal binding to nitrilotriacetic acid. Inhibition was not due to metal ion toxicity, but was a result of metal–nitrilotriacetic acid complexes being recalcitrant to degradation. In addition, the effect of complexing (phosphate) and non-complexing (PIPES) buffers on bioavailability was examined; Co and Zn prevented degradation of nitrilotriacetic acid in PIPES buffer, but not in phosphate buffer. This was due to the removal of Co and Zn from solution by phosphate precipitation, leaving nitrilotriacetic acid uncomplexed. The results demonstrated that metal–organic complexation can alter the bioavailability of organic pollutants and may also modulate the toxicity of heavy metals.