Abstract
An orange-pigmented myxobacterium, designated strain c25j21T, was isolated from subtropical forest soil collected from the Chebaling National Nature Reserve in Guangdong Province, China. Phylogenetic analysis based on the 16S rRNA gene and core genes clearly showed that the novel strain was affiliated within the genus Corallococcus and most closely related to Corallococcus aberystwythensis DSM 108846T (99.3% 16S rRNA gene sequence similarity), while C. exercitus DSM 108849T (99.2%) and C. carmarthensis DSM 108842T (99.0%) were the next most closely related type strains. The draft genome sequence of strain c25j21T was 9.23 Mb in length with a G + C content of 70.7 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain c25j21T and its closely related type strains were 88.1–89.1 and 34.1–36.3%, respectively. The major fatty acids contained iso-C15:0, iso-C17:0, iso-C17:1ω5c and iso-C17:0 2-OH. The predominant respiratory quinone was menaquinone 7. Based on phylogenetic, phenotypic and chemotaxonomic analysis, strain c25j21T represents a novel species of the genus Corallococcus, for which the name Corallococcus silvisoli sp. nov. is proposed. The type strain is c25j21T (= GDMCC 1.1387T = KCTC 62437T).
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Abbreviations
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
- RAST:
-
Rapid annotation using subsystem technology
- GGDC:
-
Genome-to-genome distance calculator
- MK-7:
-
Menaquinone 7
- NJ:
-
Neighbor-joining
- MP:
-
Maximum-parsimony
- ML:
-
Maximum likelihood
- BGCs:
-
Biosynthetic gene clusters
- PKS:
-
Polyketide synthase
- NRPS:
-
Non-ribosomal peptide synthetase cluster
- UBCG:
-
Up-to-data bacteria core
- SEM:
-
Scanning electron microscope
- DMA:
-
Dimethylacetal
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Funding
This work was jointly supported by the Key Realm R&D Program of Guangdong Province (2018B020205003), the Natural Science Foundation of China (32000007) and the GDAS' Project of Science and Technology Development (2021GDASYL-20210103020).
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Strain c25j21T was isolated by XJZ. Experiments, data collection and analysis were performed by XJZ, YL, YZ and XQD. The manuscript was written by XJZ. Project guidance and critical revision of manuscripts was performed by GDF, QY and HHZ. All authors read and approved the final manuscript.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genome sequences of strain c25j21T are MN911319 and JAAAPJ000000000, respectively.
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Communicated by Erko Stackebrandt.
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Zhang, XJ., Feng, GD., Liu, Y. et al. Corallococcus silvisoli sp. nov., a novel myxobacterium isolated from subtropical forest soil. Arch Microbiol 204, 141 (2022). https://doi.org/10.1007/s00203-021-02725-5
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DOI: https://doi.org/10.1007/s00203-021-02725-5