Abstract
Fish gut microbiota, encompassing a colossal reserve of microbes represents a dynamic ecosystem, influenced by a myriad of environmental and host factors. The current study presents a comprehensive insight into Sardinella longiceps gut microbiome using whole metagenome shotgun sequencing. Taxonomic profiling identified the predominance of phylum Proteobacteria, comprising of Photobacterium, Vibrio and Shewanella sp. Functional annotation revealed the dominance of Clustering based subsystems, Carbohydrate, and Amino acids and derivatives. Analysis of Virulence, disease and defense subsystem identified genes conferring resistance to antibiotics and toxic compounds, like multidrug resistance efflux pumps and resistance genes for fluoroquinolones and heavy metals like cobalt, zinc, cadmium and copper. The presence of overlapping genetic mechanisms of resistance to antibiotics and heavy metals, like the efflux pumps is a serious cause of concern as it is likely to aggravate co-selection pressure, leading to an increased dissemination of these resistance genes to fish and humans.
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The dataset generated during the current study is available in the NCBI BioSample Submission Portal as BioProject PRJNA433183 and SRA accession number SRR6677342.
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The authors gratefully acknowledge the project grants from Centre for Marine Living Resources and Ecology-Ministry of Earth Sciences, Government of India (no. MOES/10-MLR/2/2007 and MOES/10-MLRTD/03/2013) given to Dr. Sarita G. Bhat. Author Tina Kollannoor Johny acknowledges UGC, Government of India for research fellowship.
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All the authors made substantial contributions to the design of work and drafting of the manuscript. TKJ conceived the study, performed data analysis and drafted the manuscript. RMP contributed to data acquisition, analysis and drafting of the manuscript. SGB contributed to the interpretation of data and made critical comments for the revision of the manuscript. All the authors read and approved the final manuscript.
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Johny, T.K., Puthusseri, R.M. & Bhat, S.G. Metagenomic landscape of taxonomy, metabolic potential and resistome of Sardinella longiceps gut microbiome. Arch Microbiol 204, 87 (2022). https://doi.org/10.1007/s00203-021-02675-y
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DOI: https://doi.org/10.1007/s00203-021-02675-y