Abstract
The study evaluates the effect of Artemisia dracunculus essential oil (EO) on two pathogenic bacteria Salmonella enterica serovar Typhimurium and Staphylococcus aureus and Vero cell line. To evaluating the anti-biofilm potential of the EO, a microtiter-plate test (MtP) and scanning electron microscopy (SEM) were performed. The quorum-sensing inhibitory properties were examined by QS-related gene expression at sub-MIC concentrations of Artemisia dracunculus EO. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) test was used to determine the cytotoxicity potential of the EO against the Vero cell line and finally, the major components of the EOs were determined using Gas chromatography–mass spectrometry (GC–MS) analysis. The minimum inhibitory concentration (MIC) of the tested EO against S. Typhimurium and S. aureus were 2.5 and 1.25 μl/ml, respectively. In addition, the minimum bactericidal concentration was 5 and 2.5 μl/ml for S. Typhimurium and S. aureus, respectively. Both MtP and SEM showed an acceptable inhibitory and disruption effect of the EO on the biofilm formation at Sub-MIC concentrations. Significant downregulation of luxS, pfs, and hld genes by treatment with MIC/2 concentration of A. dracunculus EO was observed. The IC50 value of A. dracunculus EO against Vero cells was 20 μl/ml. The main detected compound using GC–MS was estragole (methyl chavicol or tarragon) (64.94%). Anti-biofilm, QSI activity, and non-toxicity of A. dracunculus EO reported for the first time in this study propose the use of these plant compounds as alternatives to antibiotics and chemical additives.
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References
Alni RH, Ghorban K, Dadmanesh M (2020) Combined effects of Allium sativum and Cuminum cyminum essential oils on planktonic and biofilm forms of Salmonella typhimurium isolates. 3 Biotech 10(7):1–10
Aumeeruddy-Elalfi Z, Ismaël IS, Hosenally M, Zengin G, Mahomoodally MF (2018) Essential oils from tropical medicinal herbs and food plants inhibit biofilm formation in vitro and are non-cytotoxic to human cells. 3 Biotech 8(9):395
Bintsis T (2017) Foodborne pathogens. AIMS Microbiol 3(3):529–563
Brunelle BW, Bearson BL, Bearson S (2015) Chloramphenicol and tetracycline decrease motility and increase invasion and attachment gene expression in specific isolates of multidrug-resistant Salmonella enterica serovar Typhimurium. Front Microb 5:801
Chaleshtori RS, Rokni N, Razavilar V, Kopaei MR (2013) The evaluation of the antibacterial and antioxidant activity of Tarragon (Artemisia dracunculus L) essential oil and its chemical composition. Jundishapur J Microbiol 6:e7877
Cortese YJ, Wagner VE, Tierney M, Devine D, Fogarty A (2018) Review of catheter-associated urinary tract infections and in vitro urinary tract models. J Healthc Eng 3:1–16
Damjanović-Vratnica B, Perović A, Šuković D, Perović S (2011) Effect of vegetation cycle on chemical content and antibacterial activity of Satureja montana L. Arch Biol Sci 63:1173–1179
Duarte A, Alves AC, Ferreira S, Silva F, Domingues FC (2015) Resveratrol inclusion complexes: antibacterial and anti-biofilm activity against Campylobacter spp. and Arcobacter butzleri. Food Res Int 77:244–250
El Gendy AN, Leonardi M, Mugnaini L, Bertelloni F, Ebani VV, Nardoni S, Mancianti F, Hendawy S, Omer E, Pistelli L (2015) Chemical composition and antimicrobial activity of essential oil of wild and cultivated Origanum syriacum plants grown in Sinai, Egypt. Ind Crops Prod 67:201–207
Hammer BK, Bassler BL (2003) Quorum sensing controls biofilm formation in Vibrio cholerae. Mol Microbiol 50:101–104
Hites RA (2016) Development of gas chromatographic mass spectrometry. Anal Chem 88(14):6955–6961
Kaplan JA (2010) Biofilm dispersal: mechanisms, clinical implications, and potential therapeutic uses. J Dent Res 89:205–218
Kim Y-G, Lee J-H, Gwon G, Kim S-I, Park JG, Lee J (2016) Essential oils and eugenols inhibit biofilm formation and the virulence of Escherichia coli O157: H7. Sci Rep 6:36377
Koh C-L, Sam C-K, Yin W-F, Tan L, Krishnan T, Chong Y, Chan K-G (2013) Plant-derived natural products as sources of anti-quorum sensing compounds. Sensors 13:6217–6228
Kolar SL et al (2013) Extracellular proteases are key mediators of Staphylococcus aureus virulence via the global modulation of virulence-determinant stability. Microbiologyopen 2:18–34
Koprivnjak T, Mlakar V, Swanson L, Fournier B, Peschel A, Weiss JP (2006) Cation-induced transcriptional regulation of the dlt operon of Staphylococcus aureus. J Bacteriol 188:3622–3630
Li X-H, Lee J-H (2017) Antibiofilm agents: a new perspective for antimicrobial strategy. J Microbiol 55:753–766
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25:402–408
Mumivand H, Babalar M, Tabrizi L, Craker LE, Shokrpour M, Hadian J (2017) Antioxidant properties and principal phenolic phytochemicals of Iranian tarragon (Artemisia dracunculus L.) accessions. Hortic Environ Biotechnol 58:414–422
Nostro A et al (2007) Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. J Med Microbiol 56:519–523
Obolskiy D, Pischel I, Feistel B, Glotov N, Heinrich M (2011) Artemisia dracunculus L. (tarragon): a critical review of its traditional use, chemical composition, pharmacology, and safety. J Agric Food Chem 59:11367–11384
Orhan-Yanıkan E, da Silva-Janeiro S, Ruiz-Rico M, Jiménez-Belenguer AI, Ayhan K, Barat JM (2019) Essential oils compounds as antimicrobial and antibiofilm agents against strains present in the meat industry. Food Control 101:29–38
O’Toole G, Kaplan HB, Kolter R (2000) Biofilm formation as microbial development. Annu Rev Microbiol 54:49–79
Paini A, Punt A, Viton F, Scholz G, Delatour T, Marin-Kuan M, Schilter B, van Bladeren PJ, Rietjens IM (2010) A physiologically based biodynamic (PBBD) model for estragole DNA binding in rat liver based on in vitro kinetic data and estragole DNA adduct formation in primary hepatocytes. Toxicol Appl Pharm 245:57–66
Phillips D (1994) DNA adducts derived from safrole, estragole and related compounds, and from benzene and its metabolites. IARC Sci Publisher, Lyon, p 131
Quassinti L et al (2013) Antioxidant and antiproliferative activity of Hypericum hircinum L. subsp. majus (Aiton) N. Robson essential oil. Nat Prod Res 27:862–868
Raeisi M, Tajik H, Razavi RS, Maham M, Moradi M, Hajimohammadi B, Naghili H, Hashemi M, Mehdizadeh T (2012) Essential oil of tarragon (Artemisia dracunculus) antibacterial activity on Staphylococcus aureus and Escherichia coli in culture media and Iranian white cheese. Iran J Microbiol 4:30
Rietjens IM, Punt A, Schilter B, Scholz G, Delatour T, van Bladeren PJ (2010) In silico methods for physiologically based biokinetic models describing bioactivation and detoxification of coumarin and estragole: implications for risk assessment. Mol Nutr Food Res 54:195–207
Roy R, Tiwari M, Donelli G, Tiwari V (2018) Strategies for combating bacterial biofilms: a focus on anti-biofilm agents and their mechanisms of action. Virulence 9:522–554
Rutherford ST, Bassler BL (2012) Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect 2:a012427
Sharifi A, Ahmadi A, Mohammadzadeh A (2018a) Streptococcus pneumoniae quorum sensing and biofilm formation are affected by Thymus daenensis, Satureja hortensis, and Origanum vulgare essential oils. Acta Microbiol Immunol Hung 65:345–359
Sharifi A, Mohammadzadeh A, Zahraei Salehi T, Mahmoodi P (2018b) Antibacterial, antibiofilm and antiquorum sensing effects of Thymus daenensis and Satureja hortensis essential oils against Staphylococcus aureus isolates. J Appl Microbiol 124:379–388
Swamy MK, Akhtar MS, Sinniah UR (2016) Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. Evid Based Complement Altern Med 2016:1–21
Taylor TA, Unakal CG (2019) Staphylococcus aureus. StatPearls Publishing, Treasure Island
Tongnuanchan P, Benjakul S (2014) Essential oils: extraction, bioactivities, and their uses for food preservation. J Food Sci 79:1231–1249
Yarwood JM, Schlievert PM (2003) Quorum sensing in Staphylococcus infections. J Clin Invest 112:1620–1625
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Conceptualization: LB, Methodology: LB, MG, SHM, and LK, Investigation: LB and MG. Writing original draft preparation: SHM, Writing—review and editing: LB. Supervision: LB. Project administration: LB, MG, SHM, and LK.
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Communicated by Erko Stackebrandt.
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Mohammadi Pelarti, S., Karimi Zarehshuran, L., Babaeekhou, L. et al. Antibacterial, anti-biofilm and anti-quorum sensing activities of Artemisia dracunculus essential oil (EO): a study against Salmonella enterica serovar Typhimurium and Staphylococcus aureus. Arch Microbiol 203, 1529–1537 (2021). https://doi.org/10.1007/s00203-020-02138-w
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DOI: https://doi.org/10.1007/s00203-020-02138-w