Abstract
Bacteriocyte-associated symbionts are essential for the health of many sap-sucking insects, such as cicadas, leafhoppers and treehoppers, etc., but little is known about the bacterial community in the gut and other related organs in these insects. We characterized the bacterial communities in the salivary glands, alimentary canal and the Malpighian tubules of two populations of the cicada Subpsaltria yangi occurring in different habitats and feeding on different hosts. A high degree of similarity of core microbiota was revealed between the two populations, both with the top three bacteria belonging to Meiothermus, Candidatus Sulcia and Halomonas. The bacterial communities in various organs clustered moderately by populations possibly reflect adaptive changes in the microbiota of related S. yangi populations, which provide a better understanding of the speciation and adaptive mechanism of this species to different diets and habitats. When compared with two phylogenetically distant cicada species, Hyalessa maculaticollis and Meimuna mongolica, the core microbiota in S. yangi was significantly different to that of these species. In addition, our results confirm that Ca. Sulcia distributes in the digestive and excretory organs besides the bacteriomes and gonads, which provide potential important information onto the trophic functions of this obligate endosymbiont to the host insects.
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Acknowledgements
We thank Mr. Zehai Hou (Northwest A&F University, China) for specimen collection; Mr. Yunxiang Liu (Northwest A&F University, China) for providing the digital elevation map. This study was supported by the National Natural Science Foundation of China (Grant Nos. 31572302, 31772505).
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Wang, D., Wei, C. Bacterial communities in digestive and excretory organs of cicadas. Arch Microbiol 202, 539–553 (2020). https://doi.org/10.1007/s00203-019-01763-4
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DOI: https://doi.org/10.1007/s00203-019-01763-4